Transcript: Human XM_005254680.3

PREDICTED: Homo sapiens WD repeat domain 76 (WDR76), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WDR76 (79968)
Length:
3990
CDS:
275..1963

Additional Resources:

NCBI RefSeq record:
XM_005254680.3
NBCI Gene record:
WDR76 (79968)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005254680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000243003 TGCACCCAACTCGGTATATTT pLKO_005 1884 CDS 100% 15.000 21.000 N WDR76 n/a
2 TRCN0000243000 CTGTCTAAGGAGCCTAGTAAT pLKO_005 2003 3UTR 100% 13.200 18.480 N WDR76 n/a
3 TRCN0000168714 GCATTCGTTTGGTGGAGAATA pLKO.1 1834 CDS 100% 13.200 18.480 N WDR76 n/a
4 TRCN0000243001 AGACAACAATGAACGATTTAA pLKO_005 862 CDS 100% 15.000 10.500 N WDR76 n/a
5 TRCN0000243004 AGTTACCACAGGCCCAATATT pLKO_005 1012 CDS 100% 15.000 10.500 N WDR76 n/a
6 TRCN0000243002 GATTGAGGGATACTCATATTT pLKO_005 1485 CDS 100% 15.000 10.500 N WDR76 n/a
7 TRCN0000167159 CCTTAGTGTGTTTATGTGGTA pLKO.1 2031 3UTR 100% 2.640 1.848 N WDR76 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005254680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04156 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04156 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000479593 AAACCAGCTGTTTCAAATCCATGC pLX_317 20.5% 100% 100% V5 n/a
4 ccsbBroadEn_08995 pDONR223 100% 89.7% 89.6% None 0_1ins192;265T>G n/a
5 ccsbBroad304_08995 pLX_304 0% 89.7% 89.6% V5 0_1ins192;265T>G n/a
6 TRCN0000465817 ACGCGACGGGTCAACGCCGTATCC pLX_317 22.4% 89.7% 89.6% V5 0_1ins192;265T>G n/a
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