Construct: ORF ccsbBroad304_09036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003053.1_s304c1, BRDN0000399496
- Derived from:
- ccsbBroadEn_09036
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR61 (80349)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09036
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80349 | WDR61 | WD repeat domain 61 | NM_001303247.2 | 100% | 100% | |
| 2 | human | 80349 | WDR61 | WD repeat domain 61 | NM_025234.3 | 100% | 100% | |
| 3 | human | 80349 | WDR61 | WD repeat domain 61 | XM_011522094.2 | 100% | 100% | |
| 4 | human | 80349 | WDR61 | WD repeat domain 61 | XM_017022637.2 | 86.9% | 79.9% | (many diffs) |
| 5 | human | 80349 | WDR61 | WD repeat domain 61 | NM_001303248.2 | 69.5% | 69.5% | 39_40ins279 |
| 6 | human | 80349 | WDR61 | WD repeat domain 61 | XR_001751403.2 | 54.5% | 1_176del;924_1308del;1477_1678del | |
| 7 | human | 80349 | WDR61 | WD repeat domain 61 | XR_931918.2 | 54.3% | 1_181del;929_1313del;1482_1683del | |
| 8 | mouse | 66317 | Wdr61 | WD repeat domain 61 | NM_001025375.2 | 92.8% | 97.3% | (many diffs) |
| 9 | mouse | 66317 | Wdr61 | WD repeat domain 61 | NM_023191.3 | 92.8% | 97.3% | (many diffs) |
| 10 | mouse | 66317 | Wdr61 | WD repeat domain 61 | NM_001025376.2 | 63.1% | 66.5% | (many diffs) |
| 11 | mouse | 66317 | Wdr61 | WD repeat domain 61 | XM_011242785.2 | 63.1% | 66.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 981
- ORF length:
- 915
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac caaccagtac ggtattctct tcaaacaaga gcaagcccat gatgatgcca 121 tttggtcagt tgcttggggg acaaacaaga aggaaaactc tgagacagtg gtcacaggct 181 ccctagatga cctggtgaag gtctggaaat ggcgtgatga gaggctggac ctacagtgga 241 gtctggaggg acatcagctg ggagtggtgt ctgtggacat cagccacacc ctgcccattg 301 ctgcatccag ctctcttgat gctcatattc gtctttggga cttggaaaat ggcaaacaga 361 taaagtccat agatgcagga cctgtggatg cctggacttt ggccttttct cctgattccc 421 agtatctggc cacaggaact catgtcggga aagtgaacat ttttggtgtg gaaagtggga 481 aaaaggaata ttctttggac acgagaggaa aattcattct tagtattgca tatagtcctg 541 atgggaaata cctagccagt ggagccatag atggaatcat caatattttt gatattgcaa 601 ctggaaaact tctgcatacc ctggaaggcc atgccatgcc cattcgctcc ttgacctttt 661 ccccggactc ccagctcctt gtcactgctt cagatgatgg ctacatcaag atctatgatg 721 tacaacatgc caatttggct ggcacgctga gcggccatgc ctcctgggtg ctgaacgttg 781 cattctgtcc tgatgacact cactttgttt ccagttcgtc tgacaaaagt gtaaaagttt 841 gggatgttgg aacgaggact tgtgttcaca ccttctttga tcaccaggat caggtctggg 901 gagtaaaata caatggaaat ggttcaaaaa ttgtgtctgt tggagatgac caggaaattc 961 acatctatga ttgtccaatt tacccaactt tcttgtacaa agtggttggt aagcctatcc 1021 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1081 ttaagtcgac aatcaacctc tggatta