Construct: ORF ccsbBroad304_09074
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004882.1_s304c1, BRDN0000408330
- Derived from:
- ccsbBroadEn_09074
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COL21A1 (81578)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09074
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318751.2 | 99.9% | 100% | 438A>G |
| 2 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_030820.4 | 99.9% | 100% | 438A>G |
| 3 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514924.2 | 99.9% | 100% | 438A>G |
| 4 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514925.3 | 99.9% | 100% | 438A>G |
| 5 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514926.1 | 99.9% | 100% | 438A>G |
| 6 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_011514927.1 | 99.9% | 100% | 438A>G |
| 7 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318752.2 | 99.6% | 99.6% | 438A>G;1278_1279insAATGGAGAG |
| 8 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_006715223.2 | 98.7% | 98.7% | 438A>G;2089_2090ins36 |
| 9 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NR_134849.2 | 68.5% | (many diffs) | |
| 10 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XR_001743657.2 | 68.3% | (many diffs) | |
| 11 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NR_134850.2 | 68.1% | (many diffs) | |
| 12 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NR_134851.2 | 67.5% | (many diffs) | |
| 13 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | XM_024446561.1 | 50.1% | 50% | 0_1ins1423;3_4insGTAAGCCT |
| 14 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318753.2 | 37% | 36.8% | 0_1ins1792;6_6delGinsACACGGGGAGAGCCT |
| 15 | human | 81578 | COL21A1 | collagen type XXI alpha 1 c... | NM_001318754.2 | 32.8% | 24.6% | 0_1ins1927;273_274insGA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2940
- ORF length:
- 2871
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcactat attacatttc tctgcatggt tttggtgctg cttcttcaga 121 attctgtgtt agctgaagat ggggaagtaa gatcaagttg tcgtactgct ccgacagatt 181 tagttttcat cttagatggc tcttatagtg ttggcccaga aaactttgaa atagtgaaaa 241 agtggcttgt caatatcaca aaaaactttg acatagggcc gaagtttatt caagttggag 301 tggttcaata tagtgactac cctgtgctgg agattcctct cggaagctat gattcaggag 361 aacatttgac ggcagcagtg gaatccatac tctacttagg aggaaacaca aagacaggga 421 aggccatcca gtttgcgctc gattaccttt ttgccaagtc ctcacgattt ctgactaaga 481 tagcagtggt acttacggat ggcaagtccc aagatgacgt caaggatgca gctcaagcag 541 caagagatag taagataaca ttatttgcta ttggtgttgg ttcagaaaca gaagatgccg 601 aacttagagc tattgccaac aagccttcgt ctacttatgt gttttatgtg gaagactata 661 ttgcaatatc caaaataagg gaagtgatga agcagaaact ttgtgaagaa tctgtctgtc 721 caacacgaat tccagtggca gctcgtgatg aaaggggatt tgatattctt ttaggtttag 781 atgtaaataa aaaggttaag aaaagaatac agctttcacc aaaaaagata aaaggatatg 841 aagtaacatc aaaagttgat ttatcagaac tcacaagcaa tgttttccca gaaggtcttc 901 ctccatcata tgtatttgtg tctactcaaa gatttaaagt caagaaaatt tgggatttat 961 ggagaatatt aactattgat ggaaggccac aaatagcagt taccttaaat ggtgtggaca 1021 aaatcttatt atttacaaca accagcgtaa ttaatggctc acaagtggtt acctttgcta 1081 accctcaagt taagacgttg tttgatgaag gctggcacca aattcgtctc ttagtaacag 1141 aacaagatgt gactttgtat attgatgacc aacaaattga aaacaagccc ttacatccag 1201 ttttagggat cttgatcaat gggcaaaccc aaattggaaa atattctgga aaagaagaaa 1261 ctgttcagtt tgatgtccaa aagttgcgaa tctactgtga cccagaacag aacaaccggg 1321 agacagcatg tgagattcct ggatttaatg gagagtgcct taatggtccc agtgatgtag 1381 gttcaactcc agctccctgt atttgtcctc cgggaaaacc aggacttcaa ggccccaaag 1441 gtgaccctgg actgcctggg aaccctggct accctggaca acctggtcaa gatggtaagc 1501 ctggatatca gggaattgca gggacaccag gtgttccagg atctccagga atacaaggag 1561 ctcgaggact accaggttac aaaggagaac cagggcgaga tggtgacaag ggtgatcgtg 1621 gacttcctgg ttttcctggg cttcatggca tgccaggatc aaagggtgaa atgggtgcca 1681 aaggagacaa aggatcacct ggattttatg gcaaaaaggg tgcaaaaggt gaaaagggga 1741 atgctggctt ccctggcctc cctggacctg ctggagaacc aggaagacat ggaaaggatg 1801 gattaatggg tagtcccggt ttcaagggag aagcaggatc ccctggtgct ccggggcagg 1861 atggaacacg gggagagcct ggaatcccag gatttcctgg aaaccgagga ttaatgggcc 1921 aaaagggaga aattgggcct ccaggacagc aaggaaaaaa aggagcccca gggatgcctg 1981 gtttaatggg aagcaatggc tcaccaggcc agcctggaac accgggatct aagggaagca 2041 aaggtgaacc tggaattcaa gggatgcctg gggcttctgg gctcaaggga gaaccaggag 2101 caacgggttc cccaggagaa ccaggataca tgggtttacc cgggattcaa ggaaaaaagg 2161 gggacaaagg aaatcaaggt gaaaaaggta ttcagggtca aaagggagaa aatggaagac 2221 agggaattcc agggcaacag ggaattcaag gccatcatgg tgcaaaagga gagagaggtg 2281 aaaagggaga acctggtgtc cgaggtgcca ttggatcaaa aggagaatct ggggtggatg 2341 gcttgatggg gcccgcaggt cctaaggggc aacctgggga tccaggtcct cagggacccc 2401 caggtttgga tgggaagccc ggaagagagt tttcagaaca atttattcga caagtttgca 2461 cagatgtaat aagagcccag ctaccagtct tacttcagag tggaagaatt agaaattgtg 2521 atcattgcct gtcccaacat ggctccccgg gtattcctgg gccacctggt ccgataggcc 2581 cagagggtcc cagaggatta cctggtttgc caggaagaga tggtgttcct ggattagtgg 2641 gtgtccctgg acgtccaggt gtcagaggat taaaaggcct accaggaaga aatggggaaa 2701 aagggagcca agggtttggg tatcctggag aacaaggtcc tcctggtccc ccaggtccag 2761 agggccctcc tggaataagc aaagaaggtc ctccaggaga cccaggtctc cctggcaaag 2821 atggagacca tggaaaacct ggaatccaag ggcaaccagg ccccccaggc atctgcgacc 2881 catcactatg ttttagtgta attgccagaa gagatccgtt cagaaaagga ccaaactatt 2941 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 3001 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 3061 ggatta