Construct: ORF ccsbBroad304_09081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012251.1_s304c1, BRDN0000405063
- Derived from:
- ccsbBroadEn_09081
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- REXO5 (81691)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09081
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81691 | REXO5 | RNA exonuclease 5 | NM_001199053.2 | 99.9% | 100% | 1680A>G |
| 2 | human | 81691 | REXO5 | RNA exonuclease 5 | NM_030941.3 | 99.9% | 100% | 1680A>G |
| 3 | human | 81691 | REXO5 | RNA exonuclease 5 | XM_005255604.2 | 99.9% | 100% | 1680A>G |
| 4 | human | 81691 | REXO5 | RNA exonuclease 5 | XM_011545963.2 | 99.9% | 100% | 1680A>G |
| 5 | human | 81691 | REXO5 | RNA exonuclease 5 | NM_001144924.2 | 95.9% | 95.9% | 1624_1625ins93 |
| 6 | human | 81691 | REXO5 | RNA exonuclease 5 | XM_017023739.2 | 95.9% | 95.9% | 1624_1625ins93 |
| 7 | human | 81691 | REXO5 | RNA exonuclease 5 | XM_011545964.2 | 95.4% | 95.4% | 1382_1383ins105;1575A>G |
| 8 | human | 81691 | REXO5 | RNA exonuclease 5 | XM_011545965.2 | 91.4% | 91.4% | 1382_1383ins105;1519_1520ins93 |
| 9 | human | 81691 | REXO5 | RNA exonuclease 5 | XR_950856.3 | 84.7% | (many diffs) | |
| 10 | human | 81691 | REXO5 | RNA exonuclease 5 | XR_002957845.1 | 81.3% | (many diffs) | |
| 11 | human | 81691 | REXO5 | RNA exonuclease 5 | XM_024450462.1 | 66.7% | 66.7% | 0_1ins771;909A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2388
- ORF length:
- 2322
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gccagagagg gaagggaccg agagacaccc caggaaggtc agggaaagca 121 ggcaggcccc aaataagctg gtcggggcag ctgaggcgat gaaagccggt tgggatctcg 181 aggagagtca gcccgaggcc aagaaagccc gcttatctac cattttattt actgacaact 241 gtgaagtaac ccatgaccag ctgtgtgaat tgctgaagta tgcagttctg ggcaaatcca 301 atgttccaaa acccagctgg tgccagcttt ttcatcaaaa ccacctaaac aacgtagtgg 361 tttttgttct gcagggaatg agtcagctac acttttacag gttctatttg gagtttggat 421 gtcttcgaaa agcattcaga cataaattcc gcttgcctcc accatcatct gattttctag 481 ctgatgttgt tgggctacaa actgaacaaa gagctggaga tctgcccaag acaatggaag 541 ggcctttacc ttctaatgca aaagccgcca tcaaccttca ggatgatccc atcattcaaa 601 agtatggctc taagaaagtg ggcttgacca gatgccttct gacaaaggag gaaatgagaa 661 cgtttcactt tccattacaa ggttttcctg attgtgaaaa ctttttactt accaaatgta 721 atggttctat agcagacaat agtcctctct ttggacttga ctgtgaaatg tgcctcacat 781 ccaaggggag agagctaaca cgcatctcac tggttgctga aggaggctgc tgtgttatgg 841 atgaactggt caaacctgaa aacaagattc tggactacct caccagcttt tcgggaatca 901 cgaagaagat tcttaaccca gtgacgacca aactcaaaga tgtacagagg cagttaaaag 961 cactgcttcc tcctgatgct gtgttagtgg gccactcctt agatttggat ctcagagcac 1021 tgaaaatgat acatccatat gttattgata catcgttgct ttatgtcaga gagcagggca 1081 gaagatttaa gctcaagttc ttagccaaag ttattttggg gaaggatata cagtgtccag 1141 acagacttgg tcatgatgcc acagaagatg ctagaacaat ccttgaattg gctcggtatt 1201 tccttaagca tggcccaaaa aagattgcag aactaaatct agaagcacta gctaatcacc 1261 aagaaataca agcagcaggc caagagccta aaaacacagc agaagtactt cagcacccaa 1321 acacaagtgt tttagaatgc ttggattcag tgggtcagaa gcttcttttt ttgacccggg 1381 agacagatgc tggtgaactt ccatcttcca gaaattgtca aactattaag tgtctttcaa 1441 ataaagaggt tcttgagcag gccagagtgg aaatccccct gtttcccttc agcattgttc 1501 agttctcttt taaggccttt tcacctgtcc tcactgagga gatgaacaaa aggatgagga 1561 tcaagtggac agagatatca actgtctatg ctgggccatt tagcaaaaat tgcaatctca 1621 gggctctgaa gaggctgttt aaaagctttg gcccagtcca gtcaatgact tttgttcttg 1681 aaacccgtca gcctcatctc tgtatacagt atgaagtcct agaagctgcc cagctggcca 1741 tagagtcctt ggatggtatt ctggtagatg gtatctgcat caaggtgcag aggcctgtga 1801 cagagctcac gcttgattgt gacaccctcg tgaatgagct ggaaggagat tctgaaaacc 1861 aaggctctat atatctgtct ggagtgagtg aaaccttcaa agaacagcta ttgcaggagc 1921 cccgcctctt tcttggcctg gaagctgtga tcttgcctaa agatcttaaa agtggaaagc 1981 agaaaaaata ctgtttcctg aaattcaaaa gttttggcag tgcccagcag gccctcaaca 2041 ttctcacagg caaggactgg aagctgaaag gcaggcatgc cctaaccccc aggcacctcc 2101 atgcctggct cagaggctta ccacctgaat caacaaggct cccagggctt cgtgttgtac 2161 ctcccccctt tgaacaggag gccttgcaga ctctgaaact ggaccacccg aagatagcag 2221 cctggcgctg gagccggaag attggaaagc tctacaacag cttgtgcccg ggcactctct 2281 gcctcatcct gctgccagga accaagagca ctcatggttc actctctggt ctaggactga 2341 tgggaataaa agaggaagaa gaaagcgctg gcccaggcct gtgttcgtgc ccaactttct 2401 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2461 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta