Construct: ORF ccsbBroad304_09118
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013792.1_s304c1, BRDN0000403820
- Derived from:
- ccsbBroadEn_09118
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SETDB2 (83852)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09118
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83852 | SETDB2 | SET domain bifurcated histo... | NM_001160308.3 | 99.8% | 99.7% | 314A>G;1381G>A;1416A>G |
| 2 | human | 83852 | SETDB2 | SET domain bifurcated histo... | NM_031915.3 | 98.1% | 98% | (many diffs) |
| 3 | human | 83852 | SETDB2 | SET domain bifurcated histo... | NM_001320699.2 | 96.5% | 96.3% | (many diffs) |
| 4 | human | 107303344 | SETDB2-PHF11 | SETDB2-PHF11 readthrough | NM_001320727.2 | 76.3% | 63.3% | (many diffs) |
| 5 | human | 107303344 | SETDB2-PHF11 | SETDB2-PHF11 readthrough | NR_135324.2 | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2187
- ORF length:
- 2121
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg agaaaaaaat ggcgatgcaa aaactttctg gatggagcta gaagatgatg 121 gaaaagtgga cttcattttt gaacaagtac aaaatgtgct gcagtcactg aaacaaaaga 181 tcaaagatgg gtctgccacc aataaagaat acatccaagc aatgattcta gtgaatgaag 241 caactataat taacagttca acatcaataa aggatcctat gcctgtgact cagaaggaac 301 aggaaaacaa atccaatgca tttccctcta catcatgtga aaactccttt ccagaagact 361 gtacatttct aacaacagga aataaggaaa ttctctctct tgaagataaa gttgtagact 421 ttagagaaaa agactcatct tcgaatttat cttaccaaag tcatgactgc tctggtgctt 481 gtctgatgaa aatgccactg aacttgaagg gagaaaaccc tctgcagctg ccaatcaaat 541 gtcacttcca aagacgacat gcaaagacaa actctcattc ttcagcactc cacgtgagtt 601 ataaaacccc ttgtggaagg agtctacgaa acgtggagga agtttttcgt tacctgcttg 661 agacagagtg taacttttta tttacagata acttttcttt caatacctat gttcagttgg 721 ctcggaatta cccaaagcaa aaagaagttg tttctgatgt ggatattagc aatggagtgg 781 aatcagtgcc catttctttc tgtaatgaaa ttgacagtag aaagctccca cagtttaagt 841 acagaaagac tgtgtggcct cgagcatata atctaaccaa cttttccagc atgtttactg 901 attcctgtga ctgctctgag ggctgcatag acataacaaa atgtgcatgt cttcaactga 961 cagcaaggaa tgccaaaact tcccccttgt caagtgacaa aataaccact ggatataaat 1021 ataaaagact acagagacag attcctactg gcatttatga atgcagcctt ttgtgcaaat 1081 gtaatcgaca attgtgtcaa aaccgagttg tccaacatgg tcctcaagtg aggttacagg 1141 tgttcaaaac tgagcagaag ggatggggtg tacgctgtct agatgacatt gacagaggga 1201 catttgtttg catttattca ggaagattac taagcagagc taacactgaa aaatcttatg 1261 gtattgatga aaacgggaga gatgagaata ctatgaaaaa tatattttca aaaaagagga 1321 aattagaagt tgcatgttca gattgtgaag ttgaagttct cccattagga ttggaaacac 1381 atcctagaac tgctaaaact gagaaatgtc caccaaagtt cagtaataat cccaaggagc 1441 ttactatgga aacgaaatat gataatattt caagaattca gtatcattca gttattagag 1501 atcctgaatc caagacagcc atttttcaac acaatgggaa aaaaatggaa tttgtttcct 1561 cggagtctgt cactccagaa gataatgatg gatttaaacc accccgagag catctgaact 1621 ctaaaaccaa gggagcacaa aaggactcaa gttcaaacca tgttgatgag tttgaagata 1681 atctgctgat tgaatcagat gtgatagata taactaaata tagagaagaa actccaccaa 1741 ggagcagatg taaccaggcg accacattgg ataatcagaa tattaaaaag gcaattgagg 1801 ttcaaattca gaaaccccaa gagggacgat ctacagcatg tcaaagacag caggtatttt 1861 gtgatgaaga gttgctaagt gaaaccaaga atacttcatc tgattctcta acaaagttca 1921 ataaagggaa tgtgttttta ttggatgcca caaaagaagg aaatgtcggc cgcttcctta 1981 atcatagttg ttgcccaaat ctcttggtac agaatgtttt tgtagaaaca cacaacagga 2041 attttccatt ggtggcattc ttcaccaaca ggtatgtgaa agcaagaaca gagctaacat 2101 gggattatgg ctatgaagct gggactgtgc ctgagaagga aatcttctgc caatgtgggg 2161 ttaataaatg tagaaaaaaa atattatacc caactttctt gtacaaagtg gttggtaagc 2221 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 2281 ggcgcgttaa gtcgacaatc aacctctgga tta