Construct: ORF ccsbBroad304_09162

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000887.1_s304c1, BRDN0000398868
Derived from:
ccsbBroadEn_09162
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FYTTD1 (84248)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09162
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84248 FYTTD1 forty-two-three domain cont... NM_032288.7 99.8% 99.6% 260G>A
2 human 84248 FYTTD1 forty-two-three domain cont... NM_001011537.2 90.2% 88.1% (many diffs)
3 human 84248 FYTTD1 forty-two-three domain cont... NR_027840.2 13% (many diffs)
4 mouse 69823 Fyttd1 forty-two-three domain cont... NM_027226.4 87.8% 92.1% (many diffs)
5 mouse 69823 Fyttd1 forty-two-three domain cont... XM_006522521.3 80.8% 84.2% (many diffs)
6 mouse 69823 Fyttd1 forty-two-three domain cont... NM_001159349.1 77.9% 81.7% (many diffs)
7 mouse 69823 Fyttd1 forty-two-three domain cont... XM_006522522.3 69.7% 72.6% (many diffs)
8 mouse 69823 Fyttd1 forty-two-three domain cont... XM_006522523.3 61.4% 61.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1020
ORF length:
954
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa ccggtttggt acccggttgg tgggagccac ggcgacttct tcgccgccgc
121cgaaggcccg cagcaatgaa aacctcgaca aaatagatat gtctttggat gatatcatca
181agttgaatcg aaaggaaggg aagaagcaga attttccaag actaaataga agactcctcc
241agcaaagtgg tgcccagcaa ttcaggatga gagtgcgatg gggaatccaa cagaattctg
301gttttggtaa gactagtctg aatcatagag gaagagtaat gcctggaaag agacgtccta
361atggagttat cactggcctt gcagctagga aaacgactgg aattcgaaaa ggaattagtc
421ctatgaatcg tccacctcta agtgacaaga atatagaaca atattttcca gtgttaaaaa
481ggaaggcaaa ccttctgaga caaaatgaag ggcagaggaa accagtagca gttctcaaga
541gacctagcca gctaagcaga aaaaataaca ttccagctaa ttttaccagg agtggaaata
601aattaaatca tcagaaagat actcgtcagg caacttttct tttcagaaga ggcctgaagg
661tgcaggccca gttgaataca gaacaactgc tagacgatgt agtagcaaag agaactcgtc
721aatggcggac ttccaccaca aatggaggga ttttgactgt atctattgac aatcctggag
781cagtgcaatg cccagtaact cagaaaccac gattaactcg tactgctgta ccttcatttt
841taacaaagcg ggagcaaagt gacgtcaaga aagttcctaa aggtgttccc ctgcagtttg
901acataaacag tgtcggaaaa cagacaggga tgacgttgaa tgagcggttt gggatcctga
961aggaacaaag agccactctc acatacaaca aagggggaag ccgctttgtc accgtgggat
1021acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1081attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1141ggatta

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