Construct: ORF ccsbBroad304_09259

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF010978.1_s304c1, BRDN0000411211
• Derived from: ccsbBroadEn_09259
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: RGS8 (85397)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_09259
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	85397	RGS8	regulator of G protein sign...	NM_033345.3	99.8%	100%	345A>C
2	human	85397	RGS8	regulator of G protein sign...	XM_011510089.3	99.8%	100%	345A>C
3	human	85397	RGS8	regulator of G protein sign...	XM_017002631.2	99.8%	100%	345A>C
4	human	85397	RGS8	regulator of G protein sign...	XM_017002632.1	99.8%	100%	345A>C
5	human	85397	RGS8	regulator of G protein sign...	XM_017002633.1	99.8%	100%	345A>C
6	human	85397	RGS8	regulator of G protein sign...	XM_017002634.1	99.8%	100%	345A>C
7	human	85397	RGS8	regulator of G protein sign...	XM_011510091.3	89.7%	89.8%	0_1ins60;285A>C
8	human	85397	RGS8	regulator of G protein sign...	NM_001102450.2	88.9%	87.3%	(many diffs)
9	human	85397	RGS8	regulator of G protein sign...	NM_001369564.2	88.9%	87.3%	(many diffs)
10	human	85397	RGS8	regulator of G protein sign...	XM_005245555.4	88.9%	87.3%	(many diffs)
11	human	85397	RGS8	regulator of G protein sign...	XM_011510090.2	88.9%	87.3%	(many diffs)
12	human	85397	RGS8	regulator of G protein sign...	XM_017002635.1	88.9%	87.3%	(many diffs)
13	human	85397	RGS8	regulator of G protein sign...	XM_017002637.2	88.9%	87.3%	(many diffs)
14	mouse	67792	Rgs8	regulator of G-protein sign...	NM_026380.3	81.5%	85.8%	(many diffs)
15	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529818.3	81.5%	85.8%	(many diffs)
16	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529819.3	81.5%	85.8%	(many diffs)
17	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529820.3	81.5%	85.8%	(many diffs)
18	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529821.3	81.5%	85.8%	(many diffs)
19	mouse	67792	Rgs8	regulator of G-protein sign...	NM_001347115.1	80.8%	87.8%	(many diffs)
20	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529822.3	80.8%	87.8%	(many diffs)
21	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529824.3	80.8%	87.8%	(many diffs)
22	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529825.3	80.8%	87.8%	(many diffs)
23	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529826.3	80.8%	87.8%	(many diffs)
24	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529827.3	80.8%	87.8%	(many diffs)
25	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529828.3	80.8%	87.8%	(many diffs)
26	mouse	67792	Rgs8	regulator of G-protein sign...	XM_006529829.3	66.1%	70.7%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

660

ORF length:

594

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgtg gaacacctta acccgaagcc tctctgacca tccagttggc aaagaccctc
121	aggccatgag gactggccaa agacagaaca aagggatgag gactcgactg ggatgcctgt
181	ctcacaagtc agactcgtgt agtgatttca cagctattct tccagacaaa cccaaccgcg
241	ctctcaagag attatcgaca gaagaagcta cgaggtgggc agattccttt gatgtgcttc
301	tctctcataa gtatggggtg gctgcattcc gtgccttctt gaagacggag ttcagtgagg
361	agaacctgga attctggttg gcctgtgagg agttcaagaa gaccaggtcc actgcaaaac
421	tggtctctaa ggcccatagg atctttgagg agtttgtgga tgtgcaggct ccacgggagg
481	taaacattga cttccagacc cgagaagcca cgaggaagaa cctgcaggag ccatccctga
541	cttgctttga ccaagcccaa ggaaaagtac acagcctcat ggagaaagac tcttacccca
601	ggttcctgag gtccaaaatg tacttagatc tgctgtccca aagccagagg aggctcagtt
661	acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
721	attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
781	ggatta

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