Construct: ORF ccsbBroad304_09283
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002000.1_s304c1, BRDN0000407875
- Derived from:
- ccsbBroadEn_09283
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM116 (89894)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09283
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_001294314.1 | 99.8% | 100% | 429A>G |
| 2 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_138341.2 | 99.8% | 100% | 429A>G |
| 3 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538957.1 | 99.8% | 100% | 429A>G |
| 4 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020190.2 | 99.8% | 100% | 429A>G |
| 5 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020192.2 | 99.8% | 100% | 429A>G |
| 6 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449265.1 | 99.8% | 100% | 429A>G |
| 7 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538958.1 | 90.4% | 90.6% | 0_1ins69;360A>G |
| 8 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449267.1 | 90.4% | 90.6% | 0_1ins69;360A>G |
| 9 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449268.1 | 90.4% | 90.6% | 0_1ins69;360A>G |
| 10 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538953.1 | 89.2% | 89.4% | 1_87del;516A>G |
| 11 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020188.2 | 89.2% | 89.4% | 1_87del;516A>G |
| 12 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449263.1 | 89.2% | 89.4% | 1_87del;516A>G |
| 13 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449264.1 | 89.2% | 89.4% | 1_87del;516A>G |
| 14 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_001193453.1 | 79.7% | 76.5% | (many diffs) |
| 15 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538947.2 | 79.7% | 76.5% | (many diffs) |
| 16 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538951.2 | 76.4% | 76.5% | 1_225del;654A>G |
| 17 | human | 89894 | TMEM116 | transmembrane protein 116 | NM_001193531.2 | 72.6% | 72.7% | 1_276del;705A>G |
| 18 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538949.2 | 72.6% | 72.7% | 1_276del;705A>G |
| 19 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449260.1 | 72.6% | 72.7% | 1_276del;705A>G |
| 20 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_006719678.3 | 67.2% | 67.3% | 1_357del;786A>G |
| 21 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449259.1 | 65.9% | 66% | 1_378del;807A>G |
| 22 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449258.1 | 63% | 63.1% | 1_429del;858A>G |
| 23 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449257.1 | 62% | 62.1% | 1_447del;876A>G |
| 24 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449266.1 | 59.2% | 46.6% | (many diffs) |
| 25 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538948.3 | 58.1% | 53.9% | (many diffs) |
| 26 | human | 89894 | TMEM116 | transmembrane protein 116 | XR_001748914.2 | 56.1% | (many diffs) | |
| 27 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_011538950.2 | 54.2% | 45.5% | (many diffs) |
| 28 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449262.1 | 54.2% | 45.5% | (many diffs) |
| 29 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_024449261.1 | 49.2% | 41.4% | (many diffs) |
| 30 | human | 89894 | TMEM116 | transmembrane protein 116 | XM_017020187.2 | 47.6% | 43.4% | (many diffs) |
| 31 | human | 89894 | TMEM116 | transmembrane protein 116 | NR_122119.1 | 38.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 801
- ORF length:
- 735
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa acacacccag agtggacaga gcacatctcc actggtgata gattatactt 121 gtcgagtttg tcaaatggcc tttgttttct caagcctgat acctctgcta ttgatgacac 181 ctgtattctg tctgggaaat actagtgaat gtttccaaaa cttcagtcag agccacaagt 241 gtatcttgat gcactcacca ccatcagcca tggctgaact tccaccttct gccaacacat 301 ctgtctgtag cacactttat ttttatggta tcgccatttt cctgggcagc tttgtactca 361 gcctccttac cattatggtc ttacttatcc gagcccagac attgtataag aagtttgtga 421 agtcaactgg ctttctgggg agtgaacagt gggcagtgat tcacattgtg gaccaacggg 481 tgcgcttcta cccggtggcc ttcttttgct gctggggccc agctgtcatt ctaatgatca 541 taaagctgac taagccacag gacaccaagc ttcacatggc cctttatgtt ctccaggctc 601 taacggcaac atctcagggt ctactcaact gtggagtata tggctggacg cagcacaaat 661 tccaccaact aaagcaggag gctcggcgtg atgcagatac ccagacacca ttattatgct 721 cacagaagag attctatagc aggggcttaa attcactgga atccaccctg acttttcctg 781 ccagtacttc taccattttt tgcccaactt tcttgtacaa agtggttggt aagcctatcc 841 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 901 ttaagtcgac aatcaacctc tggatta