Construct: ORF ccsbBroad304_09314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000610.1_s304c1, BRDN0000403831
- Derived from:
- ccsbBroadEn_09314
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SESTD1 (91404)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09314
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91404 | SESTD1 | SEC14 and spectrin domain c... | NM_178123.5 | 99.9% | 99.8% | 63A>G;146A>T |
| 2 | human | 91404 | SESTD1 | SEC14 and spectrin domain c... | XM_011512139.2 | 99.9% | 99.8% | 63A>G;146A>T |
| 3 | human | 91404 | SESTD1 | SEC14 and spectrin domain c... | XM_017005250.1 | 94% | 93.9% | 63A>G;146A>T;1521_1522ins123 |
| 4 | human | 91404 | SESTD1 | SEC14 and spectrin domain c... | XM_011512141.2 | 88.2% | 88.2% | 0_1ins246 |
| 5 | human | 91404 | SESTD1 | SEC14 and spectrin domain c... | XM_017005251.1 | 78% | 78% | 0_1ins459 |
| 6 | human | 91404 | SESTD1 | SEC14 and spectrin domain c... | XR_001739031.1 | 21.7% | (many diffs) | |
| 7 | human | 91404 | SESTD1 | SEC14 and spectrin domain c... | XR_001739032.1 | 20.7% | (many diffs) | |
| 8 | mouse | 228071 | Sestd1 | SEC14 and spectrin domains 1 | NM_175465.6 | 88.7% | 98.1% | (many diffs) |
| 9 | mouse | 228071 | Sestd1 | SEC14 and spectrin domains 1 | XM_006499200.2 | 88.7% | 98.1% | (many diffs) |
| 10 | mouse | 228071 | Sestd1 | SEC14 and spectrin domains 1 | XM_006499201.2 | 88.7% | 98.1% | (many diffs) |
| 11 | mouse | 228071 | Sestd1 | SEC14 and spectrin domains 1 | XM_006499202.3 | 88.7% | 98.1% | (many diffs) |
| 12 | mouse | 228071 | Sestd1 | SEC14 and spectrin domains 1 | XM_011239447.1 | 78.4% | 86.7% | (many diffs) |
| 13 | mouse | 228071 | Sestd1 | SEC14 and spectrin domains 1 | XM_017317597.1 | 58.2% | 64.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2154
- ORF length:
- 2088
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggcctcagta atattaccca ttctgaagaa aaaactagcc ttcctttcag 121 gaggaaagga cagacggagt ggcctcattt tgacaattcc attatgcctc gaacagacaa 181 atatggatga gctgagtgtc accttagact tcctactcag cattccaagt gagaagtgta 241 aggctagagg atttaccgtg attgtggatg gcagaaaatc acagtggaat gtggtgaaaa 301 cagtagtcgt aatgctacag aatgttgttc cagctgaggt gtcccttgtt tgtgtggtaa 361 agccagatga attctgggat aagaaagtaa cgcatttttg tttttggaag gagaaggata 421 gacttggctt tgaggttatt ttagtgtccg ccaacaaatt gactcgttat atagaaccat 481 gccaattaac agaagatttt ggtgggagtc tcacctatga tcacatggac tggttaaata 541 agaggctggt ttttgagaag tttacaaagg aatctacatc attattagat gaacttgctt 601 tgattaacaa tggaagtgat aaaggaaatc agcaagagaa agaaaggtct gtggatttaa 661 actttcttcc atcggttgat cctgaaacag ttcttcagac agggcatgaa ttgttgtccg 721 aattacagca gcgtcgattt aatggctcag acggaggggt ttcatggtct cctatggatg 781 atgaacttct tgcacagcca caggttatga aattattaga ttcactccga gagcaatata 841 cccgctacca ggaagtttgt aggcaacgta gcaagcgcac acagttagaa gagattcaac 901 agaaggtaat gcaggtggtg aactggctag aagggcctgg atcagaacaa ctaagagccc 961 agtggggcat tggagactcc attagggcct cccaggccct acagcagaaa cacgaagaga 1021 ttgagagcca gcacagtgaa tggtttgcag tgtatgtgga acttaatcag caaattgcag 1081 cactcttgaa tgctggcgat gaggaagatc ttgtggaact aaagtcactg cagcaacaac 1141 ttagtgatgt ttgttatcga caggccagtc agctggaatt taggcaaaat ctcttacaag 1201 cagctcttga atttcatggt gttgcccaag atttgtctca gcagttggat ggcttattag 1261 ggatgttgtg cgtagatgta gcaccagctg atggagcatc gattcagcaa actttaaaac 1321 tgcttgaaga gaagctgaaa agtgttgatg tgggattgca aggtttgcgt gaaaaaggtc 1381 aaggtctcct ggatcagatc tccaatcagg catcctgggc ctatggaaag gatgtaacca 1441 ttgaaaataa agaaaatgtg gaccacatac aaggagtgat ggaagatatg cagcttagaa 1501 aacaaagatg tgaagacatg gtagatgtgc gaaggttaaa gatgcttcag atggtgcagt 1561 tgtttaaatg tgaagaagat gctgcccagg cagtagaatg gctaagtgaa cttctggatg 1621 ctctgcttaa gactcacatc agattgggcg atgatgctca agaaacgaaa gttttgctgg 1681 aaaagcatag aaaatttgtt gatgttgcac agagcactta tgactatggc aggcagttgc 1741 tacaggccac agttgtgtta tgccaatctt tgcgctgcac ttctcggtca tctggggata 1801 cacttcctcg actgaacaga gtatggaaac aatttacaat agcatctgaa gagagagtac 1861 atagattgga aatggctatt gcatttcact caaatgctga aaagattttg caggactgtc 1921 cagaagagcc tgaagctatt aatgatgagg agcaatttga tgaaattgaa gcagttggga 1981 aatcactttt ggatagatta actgttccag tagtttatcc tgatggaacc gaacaatatt 2041 ttgggagtcc aagtgacatg gcttctactg cagaaaacat cagagacagg atgaaactag 2101 ttaatctcaa aaggcagcag ctgagacatc ctgaaatggt gaccacagag agctacccaa 2161 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2221 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta