Construct: ORF ccsbBroad304_09432

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014641.1_s304c1, BRDN0000408691
Derived from:
ccsbBroadEn_09432
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACSM1 (116285)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09432
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 116285 ACSM1 acyl-CoA synthetase medium ... NM_001318890.1 99.8% 100% 1362T>A;1458C>T;1647G>A
2 human 116285 ACSM1 acyl-CoA synthetase medium ... NM_052956.3 99.8% 100% 1362T>A;1458C>T;1647G>A
3 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_006721016.3 93.9% 94.1% (many diffs)
4 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_006721017.3 89.2% 89.4% (many diffs)
5 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_011545729.3 78.5% 78.5% 1361_1362delCCins371
6 human 116285 ACSM1 acyl-CoA synthetase medium ... XR_950737.3 77.5% (many diffs)
7 human 116285 ACSM1 acyl-CoA synthetase medium ... XR_243258.3 72.9% (many diffs)
8 human 116285 ACSM1 acyl-CoA synthetase medium ... XR_950738.3 72.7% (many diffs)
9 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_011545730.3 72.5% 70.7% 990_991ins307;1050_1051ins167;1173G>A
10 human 116285 ACSM1 acyl-CoA synthetase medium ... XR_001751827.2 68.1% (many diffs)
11 human 116285 ACSM1 acyl-CoA synthetase medium ... XR_429672.3 66.2% (many diffs)
12 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_011545731.2 64.8% 64.4% (many diffs)
13 human 116285 ACSM1 acyl-CoA synthetase medium ... NR_134918.1 63.3% (many diffs)
14 human 116285 ACSM1 acyl-CoA synthetase medium ... XR_001751828.2 63.1% (many diffs)
15 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_006721018.2 61.6% 61.8% (many diffs)
16 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_011545732.2 61.6% 61.8% (many diffs)
17 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_017022914.2 61.2% 57.5% (many diffs)
18 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_017022915.1 58.2% 57.3% (many diffs)
19 human 116285 ACSM1 acyl-CoA synthetase medium ... XM_024450148.1 55.8% 55.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1800
ORF length:
1731
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagtggcta atgaggttcc ggaccctctg gggcatccac aaatccttcc
121acaacatcca ccctgcccct tcacagctgc gctgccggtc tttatcagaa tttggagccc
181caagatggaa tgactatgaa gtaccggagg aatttaactt tgcaagttat gtactggact
241actgggctca aaaggagaag gagggcaaga gaggtccaaa tccagctttt tggtgggtga
301atggccaagg ggatgaagta aagtggagct tcagagagat gggagaccta acccgccgtg
361tagccaacgt cttcacacag acctgtggcc tacaacaggg agaccatctg gccttgatgc
421tgcctcgagt tcctgagtgg tggctggtgg ctgtgggctg catgcgaaca gggatcatct
481tcattcctgc gaccatcctg ttgaaggcca aagacattct ctatcgacta cagttgtcta
541aagccaaggg cattgtgacc atagatgccc ttgcctcaga ggtggactcc atagcttctc
601agtgcccctc tctgaaaacc aagctcctgg tgtctgatca cagccgtgaa gggtggctgg
661acttccgatc gctggttaaa tcagcatccc cagaacacac ctgtgttaag tcaaagacct
721tggacccaat ggtcatcttc ttcaccagtg ggaccacagg cttccccaag atggcaaaac
781actcccatgg gttggcctta caaccctcct tcccaggaag taggaaatta cggagcctga
841agacatctga tgtctcctgg tgcctgtcgg actcaggatg gattgtggct accatttgga
901ccctggtaga accatggaca gcgggttgta cagtctttat ccaccatctg ccacagtttg
961acaccaaggt catcatacag acattgttga aataccccat taaccacttt tggggggtat
1021catctatata tcgaatgatt ctgcagcagg atttcaccag catcaggttc cctgccctgg
1081agcactgcta tactggcggg gaggtcgtgt tgcccaagga tcaggaggag tggaaaagac
1141ggacgggcct tctgctctac gagaactatg ggcagtcgga aacgggacta atttgtgcca
1201cctactgggg aatgaagatc aagccgggtt tcatggggaa ggccactcca ccctacgacg
1261tccaggtcat tgatgacaag ggcagcatcc tgccacctaa cacagaagga aacattggca
1321tcagaatcaa acctgtcagg cctgtgagcc tcttcatgtg ctatgagggt gacccagaga
1381agacagctaa agtggaatgt ggggacttct acaacactgg ggacagagga aagatggatg
1441aagagggcta catttgtttc ctggggagga gtgatgacat cattaatgcc tctgggtatc
1501gcatcgggcc tgcagaggtt gaaagtgctt tggtggagca cccagcggtg gcggagtcag
1561ccgtggtggg cagcccagac ccgattcgag gggaggtggt gaaggccttt attgtcctga
1621ccccacagtt cctgtcccat gacaaggatc agctgaccaa ggaactgcag cagcatgtca
1681agtcagtgac agccccatac aagtacccaa ggaaagtgga gtttgtctca gagctgccaa
1741aaaccatcac tggcaagatt gaacggaagg aacttcggaa aaaggagact ggtcagatgt
1801tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1861attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1921ggatta

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