Transcript: Human XM_006721018.2

PREDICTED: Homo sapiens acyl-CoA synthetase medium chain family member 1 (ACSM1), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACSM1 (116285)
Length:
1411
CDS:
87..1160

Additional Resources:

NCBI RefSeq record:
XM_006721018.2
NBCI Gene record:
ACSM1 (116285)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006721018.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413792 AGTCGGAAACGGGACTAATTT pLKO_005 532 CDS 100% 15.000 21.000 N ACSM1 n/a
2 TRCN0000413404 GCGGGTTGTACAGTCTTTATC pLKO_005 279 CDS 100% 13.200 18.480 N ACSM1 n/a
3 TRCN0000045702 CTTCTGCTCTACGAGAACTAT pLKO.1 507 CDS 100% 5.625 3.938 N ACSM1 n/a
4 TRCN0000045699 CCAAGGTCATCATACAGACAT pLKO.1 322 CDS 100% 4.950 3.465 N ACSM1 n/a
5 TRCN0000045700 GACCCAGAGAAGACAGCTAAA pLKO.1 729 CDS 100% 10.800 6.480 N ACSM1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006721018.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09432 pDONR223 100% 61.6% 61.8% None (many diffs) n/a
2 ccsbBroad304_09432 pLX_304 0% 61.6% 61.8% V5 (many diffs) n/a
3 TRCN0000473697 TTTATTGACAGCAGCCAGGTGCTC pLX_317 29.2% 61.6% 61.8% V5 (many diffs) n/a
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