Construct: ORF ccsbBroad304_09468

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011299.1_s304c1, BRDN0000461869
Derived from:
ccsbBroadEn_09468
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TPH2 (121278)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09468
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 121278 TPH2 tryptophan hydroxylase 2 NM_173353.4 99.9% 99.7% 158G>A
2 mouse 216343 Tph2 tryptophan hydroxylase 2 NM_173391.3 87.3% 92.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1539
ORF length:
1470
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagccagca atgatgatgt tttccagtaa atactgggca cggagagggt
121tttccctgga ttcagcagtg cccgaagagc atcagctact tggcagctca acactaaata
181aacctaactc tggcaaaaat gacgacaaag gcaacaaggg aagcaacaaa cgtgaagctg
241ctaccgaaag tggcaagaca gcagttgttt tctccttgaa gaatgaagtt ggtggattgg
301taaaagcact gaggctcttt caggaaaaac gtgtcaacat ggttcatatt gaatccagga
361aatctcggcg aagaagttct gaggttgaaa tctttgtgga ctgtgagtgt gggaaaacag
421aattcaatga gctcattcag ttgctgaaat ttcaaaccac tattgtgacg ctgaatcctc
481cagagaacat ttggacagag gaagaagagc tagaggatgt gccctggttc cctcggaaga
541tctctgagtt agacaaatgc tctcacagag ttctcatgta tggttctgag cttgatgctg
601accacccagg atttaaggac aatgtctatc gacagagaag aaagtatttt gtggatgtgg
661ccatgggtta taaatatggt cagcccattc ccagggtgga gtatactgaa gaagaaacta
721aaacttgggg tgttgtattc cgggagctct ccaaactcta tcccactcat gcttgccgag
781agtatttgaa aaacttccct ctgctgacta aatactgtgg ctacagagag gacaatgtgc
841ctcaactcga agatgtctcc atgtttctga aagaaaggtc tggcttcacg gtgaggccgg
901tggctggata cctgagccca cgagactttc tggcaggact ggcctacaga gtgttccact
961gtacccagta catccggcat ggctcagatc ccctctacac cccagaacca gacacatgcc
1021atgaactctt gggacatgtt ccactacttg cggatcctaa gtttgctcag ttttcacaag
1081aaataggtct ggcgtctctg ggagcatcag atgaagatgt tcagaaacta gccacgtgct
1141atttcttcac aatcgagttt ggcctttgca agcaagaagg gcaactgcgg gcatatggag
1201caggactcct ttcctccatt ggagaattaa agcacgccct ttctgacaag gcatgtgtga
1261aagcctttga cccaaagaca acttgcttac aggaatgcct tatcaccacc ttccaggaag
1321cctactttgt ttcagaaagt tttgaagaag ccaaagaaaa gatgagggac tttgcaaagt
1381caattacccg tcccttctca gtatacttca atccctacac acagagtatt gaaattctga
1441aagacaccag aagtattgaa aatgtggtgc aggaccttcg cagcgacttg aatacagtgt
1501gtgatgcttt aaacaaaatg aaccaatatc tggggatttt gccaactttc ttgtacaaag
1561tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1621gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)