Transcript: Human NM_173353.4

Homo sapiens tryptophan hydroxylase 2 (TPH2), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
TPH2 (121278)
Length:
2360
CDS:
143..1615

Additional Resources:

NCBI RefSeq record:
NM_173353.4
NBCI Gene record:
TPH2 (121278)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_173353.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000056673 CCCTCTTATGAGTCTCATTTA pLKO.1 1915 3UTR 100% 13.200 9.240 N TPH2 n/a
2 TRCN0000120055 CCACGTGCTATTTCTTCACAA pLKO.1 1206 CDS 100% 4.950 3.465 N Tph2 n/a
3 TRCN0000056674 CCTCGGAAGATCTCTGAGTTA pLKO.1 605 CDS 100% 4.950 3.465 N TPH2 n/a
4 TRCN0000056675 GCTCAACACTAAATAAACCTA pLKO.1 240 CDS 100% 3.000 2.100 N TPH2 n/a
5 TRCN0000056677 GCTGACTAAATACTGTGGCTA pLKO.1 877 CDS 100% 2.640 1.848 N TPH2 n/a
6 TRCN0000056676 CCTACTTTGTTTCAGAAAGTT pLKO.1 1395 CDS 100% 0.563 0.394 N TPH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_173353.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09468 pDONR223 100% 99.9% 99.7% None 158G>A n/a
2 ccsbBroad304_09468 pLX_304 0% 99.9% 99.7% V5 158G>A n/a
3 TRCN0000478043 GCCCATCTCTAATTTGCTTACGCG pLX_317 30.9% 99.9% 99.7% V5 158G>A n/a
4 ccsbBroadEn_13081 pDONR223 100% 98.6% 98.7% None 105_106insGCTTCGACATTCCTGAAG;936A>G;1125A>T n/a
5 ccsbBroad304_13081 pLX_304 0% 98.6% 98.7% V5 105_106insGCTTCGACATTCCTGAAG;936A>G;1125A>T n/a
6 TRCN0000469246 GCCAAAGGGTCAGTCTTAGTGCCA pLX_317 25.8% 98.6% 98.7% V5 105_106insGCTTCGACATTCCTGAAG;936A>G;1125A>T n/a
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