Transcript: Mouse NM_173391.3

Mus musculus tryptophan hydroxylase 2 (Tph2), mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Tph2 (216343)
Length:
2626
CDS:
134..1600

Additional Resources:

NCBI RefSeq record:
NM_173391.3
NBCI Gene record:
Tph2 (216343)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_173391.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000056674 CCTCGGAAGATCTCTGAGTTA pLKO.1 590 CDS 100% 4.950 6.930 N TPH2 n/a
2 TRCN0000120054 GCACTTAGACTATTCCAGGAA pLKO.1 365 CDS 100% 2.640 3.696 N Tph2 n/a
3 TRCN0000120055 CCACGTGCTATTTCTTCACAA pLKO.1 1191 CDS 100% 4.950 3.960 N Tph2 n/a
4 TRCN0000120052 GCAGTCAATGTTATCCAACAT pLKO.1 1655 3UTR 100% 0.495 0.396 N Tph2 n/a
5 TRCN0000238408 ATGTGGCCATGGGCTATAAAT pLKO_005 714 CDS 100% 15.000 10.500 N Tph2 n/a
6 TRCN0000238405 TGCCGACCATCCAGGATTTAA pLKO_005 655 CDS 100% 15.000 10.500 N Tph2 n/a
7 TRCN0000238404 TCGACAGAGGAGGAAGTATTT pLKO_005 688 CDS 100% 13.200 9.240 N Tph2 n/a
8 TRCN0000238406 GTATGGAGCAGGGTTACTTTC pLKO_005 1252 CDS 100% 10.800 7.560 N Tph2 n/a
9 TRCN0000120056 GAATGCCTAATCACCACCTTT pLKO.1 1352 CDS 100% 4.950 3.465 N Tph2 n/a
10 TRCN0000120053 GCAGAGCATTGAAATTCTGAA pLKO.1 1480 CDS 100% 4.950 3.465 N Tph2 n/a
11 TRCN0000238407 TACCCGTCCCTTCTCGGTATA pLKO_005 1444 CDS 100% 0.000 0.000 N Tph2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_173391.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09468 pDONR223 100% 87.3% 92.3% None (many diffs) n/a
2 ccsbBroad304_09468 pLX_304 0% 87.3% 92.3% V5 (many diffs) n/a
3 TRCN0000478043 GCCCATCTCTAATTTGCTTACGCG pLX_317 30.9% 87.3% 92.3% V5 (many diffs) n/a
4 ccsbBroadEn_13081 pDONR223 100% 86.7% 92.1% None (many diffs) n/a
5 ccsbBroad304_13081 pLX_304 0% 86.7% 92.1% V5 (many diffs) n/a
6 TRCN0000469246 GCCAAAGGGTCAGTCTTAGTGCCA pLX_317 25.8% 86.7% 92.1% V5 (many diffs) n/a
Download CSV