Construct: ORF ccsbBroad304_09491
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011623.1_s304c1, BRDN0000404591
- Derived from:
- ccsbBroadEn_09491
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOXHD1 (125336)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09491
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | NM_001145473.2 | 99.9% | 99.8% | 1010C>T |
| 2 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_011525811.2 | 99.9% | 99.8% | 1010C>T |
| 3 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_011525810.2 | 96.7% | 96.5% | 1_51del;1061C>T |
| 4 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | NM_001173129.1 | 89.1% | 89% | 1010C>T;1371_1372ins165 |
| 5 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_024451086.1 | 47.9% | 47.9% | 1_1662del;2672C>T |
| 6 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_024451087.1 | 47.9% | 47.9% | 1_1662del;2672C>T |
| 7 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_024451088.1 | 47.9% | 47.9% | 1_1662del;2672C>T |
| 8 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_006722391.3 | 44.7% | 44.6% | 1_1896del;2906C>T |
| 9 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_024451085.1 | 44% | 44% | 1_1947del;2957C>T |
| 10 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_006722389.3 | 44% | 43.9% | 1_1950del;2960C>T |
| 11 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_006722390.3 | 44% | 43.9% | 1_1950del;2960C>T |
| 12 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | NM_001308013.1 | 42.8% | 42.7% | 1_1662del;2672C>T;3033_3034ins165 |
| 13 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_006722388.3 | 42.4% | 42.3% | 1_2082del;3092C>T |
| 14 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | NM_001145472.3 | 39.5% | 39.2% | (many diffs) |
| 15 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_011525804.2 | 30.8% | 30.7% | 1_3444del;4454C>T |
| 16 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_024451084.1 | 28.9% | 28.9% | 1_3765del;4775C>T |
| 17 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | XM_017025548.1 | 24.7% | 24.7% | 1_4665del;5675C>T |
| 18 | human | 125336 | LOXHD1 | lipoxygenase homology domai... | NM_144612.6 | 23.1% | 23.1% | 1_5097del;6107C>T |
| 19 | mouse | 240411 | Loxhd1 | lipoxygenase homology domai... | NM_172834.2 | 21.9% | 23.8% | (many diffs) |
| 20 | mouse | 240411 | Loxhd1 | lipoxygenase homology domai... | XM_006526434.1 | 19.9% | 21.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1602
- ORF length:
- 1536
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac ggtgtggaca ggggatgtgg ttggcggggg cactgactcc aacatcttca 121 tgaccctcta cggcatcaac gggagcacag aggagatgca gctggacaaa aagaaagcca 181 ggtttgagcg ggagcagaac gacaccttca tcatggagat cctagacatt gctccattca 241 ccaagatgcg gatccggatt gatggcctgg gcagtcggcc ggagtggttc ctggagagga 301 tcctactgaa gaacatgaac actggagacc tgaccatgtt ctactatgga gactggctgt 361 cccagcggaa gggcaagaag accctggtgt gtgaaatgtg tgccgttatc gatgaggaag 421 aaatgatgga gtggacctcc tacaccgtcg cagttaagac cagcgacatc ctgggagcag 481 gcactgatgc caacgtgttc atcatcatct tcggggagaa cggggatagt gggacactgg 541 ccctgaagca gtcggcaaac tggaacaagt ttgagcggaa caacacggac acattcaact 601 tccctgacat gctgagcttg ggccacctct gcaagctgag ggtctggcac gacaacaaag 661 ggatatttcc tggctggcat ctgagctatg tcgatgtgaa ggacaactcc cgcgacgaga 721 ccttccactt ccagtgtgac tgctggctct ccaagagtga gggtgacggg cagacggtcc 781 gcgactttgc ctgtgccaac aacaagatct gtgatgagct ggaagagacc acctacgaga 841 tcgtcataga aacgggcaac ggaggcgaaa ccagggagaa cgtctggctc atcctggagg 901 gcaggaagaa ccgatccaaa gagtttctca tggaaaattc ttctaggcag cgggccttta 961 ggaaggggac cacagacacg tttgagtttg acagcatcta cttgggggac attgcctccc 1021 tctgtgtggg ccaccttgcc agggaagacc ggtttatccc caagagagaa cttgtctggc 1081 atgtcaagac catcaccatc accgagatgg agtacggcaa tgtgtacttc tttaactgtg 1141 actgcctcat ccccctcaag aggaagagga agtacttcaa ggtattcgag gttaccaaga 1201 cgacagagag ctttgccagc aaggtccaga gcctggtgcc cgtcaagtac gaagtcatcg 1261 tgacaacagg ctatgagcca ggggcaggca ctgatgccaa cgtcttcgtg accatctttg 1321 gggccaacgg agacacaggc aagcgggagc tgaagcagaa aatgcgcaac ctcttcgagc 1381 ggggcagcac agaccgcttc ttcctggaga cgctggagct gggtgagctg cgcaaggtgc 1441 gcctggagca cgacagcagt ggctactgct caggctggct ggtggagaag gtggaggtca 1501 ccaacaccag caccggcgtg gccaccatct tcaactgtgg caggtggctg gacaagaagc 1561 ggggggatgg actcacctgg agagacctct tcccttctgt ctgcccaact ttcttgtaca 1621 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1681 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta