Construct: ORF ccsbBroad304_09611

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004369.1_s304c1, BRDN0000462364
Derived from:
ccsbBroadEn_09611
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
E2F7 (144455)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09611
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 144455 E2F7 E2F transcription factor 7 NM_203394.3 99.8% 99.7% 214T>C;969A>G;2721C>A
2 human 144455 E2F7 E2F transcription factor 7 XM_011537966.2 94.9% 94.7% (many diffs)
3 human 144455 E2F7 E2F transcription factor 7 XM_011537969.2 85.9% 86.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2802
ORF length:
2733
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaggtaaat tgtttaacac taaaagacct gatcagcccc aggcagccca
121gactagattt tgcagttgaa gatggggaaa atgcacaaaa ggaaaatata tttgttgatc
181gatcaaggat ggccccgaag actccaataa aaaatgaacc aattgattta tcgaagcaaa
241aaaaatttac tccagaaaga aatcccatta ctccagttaa gcttgttgac agacagcaag
301cggaaccatg gacacccaca gctaacctga agatgctcat tagtgctgcc agcccagata
361taagggaccg ggagaagaaa aagggactat tccgacccat tgaaaacaag gacgatgcat
421ttacagattc tctacagctt gatgttgttg gggacagtgc tgtggacgaa tttgaaaagc
481aaaggccaag cagaaaacag aaaagtttag gactcctgtg ccagaagttt ctagctcgct
541atccaagtta tcccttgtca actgagaaaa ctaccatctc cctagatgaa gttgctgtca
601gtcttggtgt ggaaaggaga cgcatctatg acattgtaaa tgtgctggag tcgctgcatc
661tggtcagccg ggtggctaag aatcagtatg gctggcatgg acggcacagc ctgccaaaaa
721ccctgaggaa cctccagaga ctaggagagg agcagaaata tgaagagcaa atggcctacc
781tccaacagaa agagctggac ctgatagatt ataaatttgg agaacgtaaa aaagatggtg
841atccagattc ccaggaacaa cagttactgg atttctctga acccgactgt ccctcttcat
901ctgcaaacag tagaaaagac aagtctctga gaattatgag ccagaagttt gtcatgctgt
961tcctcgtctc caaaaccaag attgtcactc tggatgtggc tgccaaaata ctgatagaag
1021aaagccaaga tgccccggac catagtaaat ttaaaacaaa ggtacgacgc ctctatgaca
1081tagccaatgt tctgaccagc ttggctctga taaagaaagt gcatgtaaca gaagagcgag
1141gtcgtaaacc agccttcaag tggatcgggc ctgtggactt cagctcaagt gatgaagaac
1201tggtggatgt ttctgcatct gtcttaccag aattgaaaag agaaacatat ggccagattc
1261aagtctgtgc aaaacagaag ctggctcgcc atggttcttt taacacagtt caggcttctg
1321agaggatcca gaggaaagtg aactcagaac cgagcagccc gtacagagaa gaacaaggat
1381caggtggcta ctctttagaa attggaagcc tggcagctgt ctatagacag aaaatagaag
1441acaattcaca gggaaaagcc tttgccagta agagagtggt gcctccatca agcagcttgg
1501accctgttgc tcctttccct gtcctctctg ttgacccaga atattgtgtt aatcctttag
1561cccacccagt attttctgtt gctcagacgg acctgcaggc attctccatg cagaacggtc
1621tgaatggaca agtggatgtc tcacttgctt ctgcagcctc tgctgtggag agcctgaagc
1681cagcactcct tgctggccag cctctagtgt atgtgccctc tgcctcactg ttcatgctgt
1741atggaagtct gcaggaggga ccagcgtcag ggtcagggtc agagagggat gacagaagct
1801cagaagcccc agccacagta gagctgtcat ctgcaccctc agctcagaag cgcctctgtg
1861aggagaggaa acctcaggag gaggatgagc cagccactaa aaggcaaagt agggaatatg
1921aagacggccc gctgtcgctt gtcatgccca agaaaccctc agattccaca gaccttgcct
1981ctcccaagac tatgggtaac agggcatcta tacccctcaa agacattcat gtgaatggcc
2041aactccctgc tgcagaagag atttcaggaa aggcaacagc aaactctctt gtttcttctg
2101agtggggaaa tccttcaaga aatacagatg ttgaaaagcc ttcaaaagaa aatgaaagca
2161ccaaagagcc ttctttgcta caatatcttt gtgtgcagtc tcctgcagga ttaaatggtt
2221tcaatgtact tttatctggc agtcaaaccc cccctactgt gggcccgtcc tcaggtcagc
2281tgccgtcttt cagtgtccct tgcatggtct taccatctcc acctctgggc ccttttcctg
2341ttctctattc tcctgcaatg ccgggcccgg tttcttctac tcttggtgct ctcccaaaca
2401caggacctgt gaatttcagc ttgcctggcc ttggatcaat agcccagctt ctcgtcggcc
2461ccacagctgt ggttaatcca aagtcgtcca cactcccttc tgcagaccct cagcttcaga
2521gtcagccctc actaaaccta agtccagtga tgtcaaggtc acacagtgtc gtccaacaac
2581ctgagtcccc cgtttacgtg ggacatccag tctcagtagt aaaattacat cagtcaccag
2641ttccagtgac ccccaagagc atccaacgca cacatcgtga gacgtttttc aagacacccg
2701gcagccttgg agaccctgtc ctgaagagaa gagaaaggaa ccagtcacga aacaccagct
2761cggcccagag gagactagaa atccccagag gcggcgctga cttgccaact ttcttgtaca
2821aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2881ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)