Construct: ORF ccsbBroad304_09616

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012387.1_s304c1, BRDN0000398864
Derived from:
ccsbBroadEn_09616
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RDH12 (145226)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09616
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 145226 RDH12 retinol dehydrogenase 12 NM_152443.3 99.8% 99.6% 482G>A
2 mouse 77974 Rdh12 retinol dehydrogenase 12 NM_030017.4 83% 86% (many diffs)
3 mouse 77974 Rdh12 retinol dehydrogenase 12 NM_001313971.1 79.7% 82.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1014
ORF length:
948
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ggtcaccttg ggactgctca cctccttctt ctcgttcctg tatatggtag
121ctccatccat caggaagttc tttgctggtg gagtgtgtag aacaaatgtg cagcttcctg
181gcaaggtagt ggtgatcact ggcgccaaca cgggcattgg caaggagacg gccagagagc
241tcgctagccg aggagcccga gtctatattg cctgcagaga tgtactgaag ggggagtctg
301ctgccagtga aatccgagtg gatacaaaga actcccaggt gctggtgcgg aaattggacc
361tatccgacac caaatctatc cgagcctttg ctgagggctt tctggcagag gaaaagcagc
421tccatattct gatcaacaat gcgggagtaa tgatgtgtcc atattccaag acagctgatg
481gctttgaaac ccacctggga gtcaaccacc tgggccactt cctcctcacc tacctgctcc
541tggagcagct aaaggtgtct gcccctgcac gggtggttaa tgtgtcctcg gtggctcacc
601acattggcaa gattcccttc cacgacctcc agagcgagaa gcgctacagc aggggttttg
661cctattgcca cagcaagctg gccaatgtgc tttttactcg tgagctggcc aagaggctcc
721aaggcaccgg ggtcaccacc tacgcagtgc acccaggcgt cgtccgctct gagctggtcc
781ggcactcctc cctgctctgc ctgctctggc ggctcttctc cccctttgtc aagacggcac
841gggagggggc gcagaccagc ctgcactgcg ccctggctga gggcctggag cccctgagtg
901gcaagtactt cagtgactgc aagaggacct gggtgtctcc aagggcccga aataacaaaa
961cagctgagcg cctatggaat gtcagctgtg agcttctagg aatccggtgg gagtacccaa
1021ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1081cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)