Construct: ORF ccsbBroad304_09621

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010570.1_s304c1, BRDN0000400796
Derived from:
ccsbBroadEn_09621
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PTGR2 (145482)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09621
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 145482 PTGR2 prostaglandin reductase 2 NM_001146154.2 99.9% 100% 228A>T
2 human 145482 PTGR2 prostaglandin reductase 2 NM_001146155.3 99.9% 100% 228A>T
3 human 145482 PTGR2 prostaglandin reductase 2 NM_001371325.1 99.9% 100% 228A>T
4 human 145482 PTGR2 prostaglandin reductase 2 NM_152444.4 99.9% 100% 228A>T
5 human 145482 PTGR2 prostaglandin reductase 2 NM_001371326.1 83.6% 83.7% 228A>T;346_347ins171
6 human 145482 PTGR2 prostaglandin reductase 2 NM_001371327.1 79.9% 80% 228A>T;516_517ins210
7 human 145482 PTGR2 prostaglandin reductase 2 NM_001371328.1 79.9% 80% 228A>T;516_517ins210
8 human 145482 PTGR2 prostaglandin reductase 2 NM_001371329.1 79.9% 80% 228A>T;516_517ins210
9 human 145482 PTGR2 prostaglandin reductase 2 NM_001371330.1 61.8% 61.8% 0_1ins402
10 human 145482 PTGR2 prostaglandin reductase 2 NM_001371331.1 61.8% 61.8% 0_1ins402
11 human 145482 PTGR2 prostaglandin reductase 2 NM_001371332.1 61.8% 61.8% 0_1ins402
12 human 145482 PTGR2 prostaglandin reductase 2 NM_001371334.1 34% 33.3% (many diffs)
13 human 145482 PTGR2 prostaglandin reductase 2 NM_001371335.1 16.6% 15.1% (many diffs)
14 mouse 77219 Ptgr2 prostaglandin reductase 2 NM_001252625.1 85.4% 86.6% (many diffs)
15 mouse 77219 Ptgr2 prostaglandin reductase 2 NM_029880.3 85.4% 86.6% (many diffs)
16 mouse 77219 Ptgr2 prostaglandin reductase 2 NM_001252626.1 72.9% 73.5% (many diffs)
17 mouse 77219 Ptgr2 prostaglandin reductase 2 XM_006516369.1 70.5% 71.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1119
ORF length:
1053
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat tgttcaaaga gtggtattga attctcgacc tggaaaaaat ggtaatccag
121tggcagagaa tttccgaatg gaagaagtct atttaccaga taatattaat gaaggacaag
181tacaagttag aactctttat ctttctgtgg atccttacat gcgttgtaga atgaatgaag
241acactggcac tgattatata acaccttggc agctatctca agtcgttgat ggtggaggta
301ttggaattat agaagaaagc aaacacacaa atttgactaa aggcgatttt gtgacttctt
361tctattggcc ctggcaaacc aaggttattc tggatggaaa tagccttgaa aaggtagacc
421cacaacttgt ggatggacac ctttcatatt ttcttggagc tataggtatg cctggtttga
481cttccttgat tgggatacag gaaaaaggtc atataactgc tggatctaat aagacaatgg
541ttgtcagtgg ggccgcaggt gcctgtggat ctgtggctgg gcagattggc catttcttag
601gttgttccag agtggtggga atttgtggaa cacatgagaa atgcatcctc ttgacctcag
661aactgggctt tgatgctgca attaattata aaaaagacaa tgtggcagaa cagctccgtg
721aatcatgccc agctggagtg gatgtttatt ttgacaatgt tggtggtaac atcagtgata
781cagtgataag tcagatgaat gagaacagcc acatcatcct gtgtggtcaa atttctcagt
841acaacaaaga tgtgccttat cctcccccgc tatcccctgc tatagaggca atccagaaag
901aaagaaacat cacaagggaa agatttctgg tattaaatta taaagacaaa tttgagcctg
961gcattctaca gctgagtcag tggtttaaag aaggaaagct aaagattaaa gagacggtaa
1021taaatgggtt ggaaaacatg ggagctgcat tccagtccat gatgacagga ggtaacattg
1081gaaagcagat agtttgcatt tcagaagaaa tctctttgta cccaactttc ttgtacaaag
1141tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1201gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)