Construct: ORF ccsbBroad304_09671

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014486.1_s304c1, BRDN0000411837
Derived from:
ccsbBroadEn_09671
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMYD1 (150572)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09671
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 150572 SMYD1 SET and MYND domain contain... NM_198274.4 99.9% 100% 337C>A
2 human 150572 SMYD1 SET and MYND domain contain... NM_001330364.2 97.2% 97.1% 337C>A;659_660ins39
3 mouse 12180 Smyd1 SET and MYND domain contain... NM_001160127.1 89.5% 94.4% (many diffs)
4 mouse 12180 Smyd1 SET and MYND domain contain... NM_009762.2 87% 91.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1539
ORF length:
1470
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gacaataggg agaatggaga acgtggaggt cttcaccgct gagggcaaag
121gaaggggtct gaaggccacc aaggagttct gggctgcaga tatcatcttt gctgagcggg
181cttattccgc agtggttttt gacagccttg ttaattttgt gtgccacacc tgcttcaaga
241ggcaggagaa gctccatcgc tgtgggcagt gcaagtttgc ccattactgc gaccgcacct
301gccagaagga tgcttggctg aaccacaaga atgaatgttc ggccatcaag agatatggga
361aggtgcccaa tgagaacatc aggctggcgg cgcgcatcat gtggagggtg gagagagaag
421gcaccgggct cacggagggc tgcctggtgt ccgtggacga cttgcagaac cacgtggagc
481actttgggga ggaggagcag aaggacctgc gggtggacgt ggacacattc ttgcagtact
541ggccgccgca gagccagcag ttcagcatgc agtacatctc gcacatcttc ggagtgatta
601actgcaacgg ttttactctc agtgatcaga gaggcctgca ggccgtgggc gtaggcatct
661tccccaacct gggcctggtg aaccatgact gttggcccaa ctgtactgtc atatttaaca
721atggcaatca tgaggcagtg aaatccatgt ttcataccca gatgagaatt gagctccggg
781ccctaggcaa gatctcagaa ggagaggagc tgactgtgtc ctatattgac ttcctcaacg
841ttagtgaaga acgcaagagg cagctgaaga agcagtacta ctttgactgc acatgtgaac
901actgccagaa aaaactgaag gatgacctct tcctgggggt gaaagacaac cccaagccct
961ctcaggaagt ggtgaaggag atgatacaat tctccaagga tacattggaa aagatagaca
1021aggctcgttc cgagggtttg tatcatgagg ttgtgaaatt atgccgggag tgcctggaga
1081agcaggagcc agtgtttgct gacaccaaca tctacatgct gcggatgctg agcattgttt
1141cggaggtcct ttcctacctc caggcctttg aggaggcctc gttctatgcc aggaggatgg
1201tggacggcta tatgaagctc taccacccca acaatgccca actgggcatg gccgtgatgc
1261gggcagggct gaccaactgg catgctggta acattgaggt ggggcacggg atgatctgca
1321aagcctatgc cattctcctg gtgacacacg gaccctccca ccccatcact aaggacttag
1381aggccatgcg ggtgcagacg gagatggagc tacgcatgtt ccgccagaac gaattcatgt
1441actacaagat gcgcgaggct gccctgaaca accagcccat gcaggtcatg gccgagccca
1501gcaatgagcc atccccagct ctgttccaca agaagcaatt gccaactttc ttgtacaaag
1561tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1621gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)