Construct: ORF ccsbBroad304_09737

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003255.1_s304c1, BRDN0000461355
Derived from:
ccsbBroadEn_09737
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPRED1 (161742)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09737
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 161742 SPRED1 sprouty related EVH1 domain... NM_152594.3 99.8% 100% 291G>A;1044T>C
2 human 161742 SPRED1 sprouty related EVH1 domain... XM_005254202.3 97.2% 97.1% 32_67del;327G>A;1080T>C
3 human 161742 SPRED1 sprouty related EVH1 domain... XM_011521289.3 95.1% 95.2% 0_1ins63;228G>A;981T>C
4 mouse 114715 Spred1 sprouty protein with EVH-1 ... NM_033524.3 91% 93% (many diffs)
5 mouse 114715 Spred1 sprouty protein with EVH-1 ... NM_001277256.1 45.1% 42% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1401
ORF length:
1332
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gagcgaggag acggcgactt ctgacaacga taatagttat gcacgagtgc
121gagctgtggt gatgacccga gatgactcaa gtggtggatg gttaccactt ggagggagtg
181gactaagcag cgtcactgtc ttcaaagtcc ctcatcagga agagaatggc tgtgctgact
241tttttatccg tggagagcga ctcagggaca aaatggtggt tttggaatgt atgcttaaaa
301aagacctcat ttataataag gtcactccaa catttcacca ctggaagatt gatgacaaaa
361agtttggtct tacgtttcaa agtcctgctg atgctagggc ttttgataga ggtatccgaa
421gagctataga ggatatttct caaggatgcc ccgaatcaaa aaatgaagct gaaggggcag
481atgacttaca agcaaatgaa gaggattctt ccagttctct agtgaaggat cacctttttc
541agcaagagac agttgttacc agtgagcctt atagaagctc aaatataaga ccttctccct
601ttgaagatct gaatgccaga agagtctaca tgcaaagcca agccaatcag ataacatttg
661gtcagccagg cttggacatt cagagcagaa gtatggaata cgtacagcgg caaatatcca
721aggaatgtgg aagcctaaag tcccaaaata gggtcccttt gaaatcaatc agacatgtca
781gctttcaaga tgaggatgag attgtcagaa taaaccctcg agatatctta atacgtcgct
841atgcagacta cagacatcct gacatgtgga aaaatgactt ggaaagagat gatgctgatt
901ccagtattca gttttctaaa ccagacagta aaaaatcaga ctatctgtac tcttgtgggg
961atgagactaa gttaagttca cccaaagact ctgtggtatt taagacgcag ccttcctcat
1021taaaaattaa gaagtcaaaa cgaagaaaag aggatggtga acgttctcgc tgcgtatact
1081gccaggaaag gtttaatcat gaagaaaatg tcaggggaaa atgtcaggat gctccagacc
1141ctattaaaag atgcatatat caagttagtt gcatgctctg tgcagagagc atgttgtatc
1201attgtatgtc agactcagag ggagattttt ctgatccctg ttcgtgtgac actagcgacg
1261acaagttctg cttgcgatgg ttagccctgg tagctttgtc tttcattgta ccatgtatgt
1321gctgctacgt ccctttgaga atgtgccatc gctgtggtga ggcatgtggt tgctgtggtg
1381ggaaacataa agctgctgga ttgccaactt tcttgtacaa agtggttggt aagcctatcc
1441ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1501ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)