Construct: ORF ccsbBroad304_09743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012582.1_s304c1, BRDN0000400059
- Derived from:
- ccsbBroadEn_09743
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FBXO39 (162517)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09743
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162517 | FBXO39 | F-box protein 39 | NM_153230.3 | 99.8% | 39.8% | 529G>T;1086C>T |
| 2 | mouse | 628100 | Fbxo39 | F-box protein 39 | NM_001099688.2 | 83.8% | 34% | (many diffs) |
| 3 | mouse | 628100 | Fbxo39 | F-box protein 39 | XM_006533902.3 | 83.8% | 34% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cgaagaaagt gaactgatcc agccccaaga ccagagctgc tgggcctttc 121 tgcccgattt gtgtctgtgc cgtgttttct ggtggctagg agacagggac aggtccaggg 181 ctgctcttgt ctgcagaaag tggaaccaga tgatgtattc tgctgagctc tggcggtaca 241 gaaccatcac cttcagcggg agaccttcca gggtacatgc atctgaagtt gagtcagctg 301 tttggtatgt taagaagttt ggtcgttatc tggagcacct ggaggtcaaa ttcatgaatc 361 cttacaatgc tgtcttgacc aagaagttcc aggtcaccat gcggggcctc ctgtcttgtc 421 tgagtaagag caacaaccgt ctgaaatctc tttccatcca atacctggag ctggaccgcc 481 tggtatggag gaacagcatc aggagctcat tcatcagcag cttgagcttc ttcttaaaga 541 agatgggcaa acgcctggat tatctcaacc taaaaggggc caggctgacc gtgtagcaag 601 gctgccaaat tctcgactcc ctcagctaca tgaggaatga gaatgtgatc tcagagctca 661 acatcgagga ctatttcagc catcaccttg ctgtctacaa cagcccccag ttcaaaaaga 721 ccatgtccac attccacaat cttgtgtccc tgaacctcaa ctacaactgt atctccgacg 781 agctgcttga gaacttgtgt gagaatgcca gcaccctccg gaccatcaac atcaaatgcc 841 acgttcatga cccccacgga caggtcatct ggggtatgtc ctgggccaag ctggccaggc 901 aggccaccaa tctgaaggtg aacttcttct ttgaacggat catgaagtac gaacgcttgg 961 cccgaatcct cttgcaggag atcccgatca ggagcatcag tctgagaagc tgctatttca 1021 gtgacccaga ctgttcaatg agacccactc tgatagatct cctgcccacc ttccggcaca 1081 ctctgcagaa attaacttgt gaattcaaca acaaccatga gtcactcgac gaggagctgc 1141 acctcctcat tatatcctgc aggaagttgt tttacttcaa aatctgggct ttccttgatg 1201 ttagttttgt ggagcggatc ctgaagagtc agaaagaacg gcagtgtgcc ctgcgtgtat 1261 tcaaggcgag aatttataca aacagatatg agacgaatga agaggacaag accctgcagg 1321 aaatttacag gaagtacaga aagctgatcg aatcagagct tagctatttt gtcatcgttt 1381 actctgtgat gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1441 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1501 caatcaacct ctggatta