Construct: ORF ccsbBroad304_09755

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013426.1_s304c1, BRDN0000407770
Derived from:
ccsbBroadEn_09755
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CNST (163882)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09755
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 163882 CNST consortin, connexin sorting... NM_001139459.1 99.9% 99.8% 260T>C
2 human 163882 CNST consortin, connexin sorting... NM_152609.3 84.7% 84.4% (many diffs)
3 human 163882 CNST consortin, connexin sorting... XM_005273081.3 84.7% 84.4% (many diffs)
4 human 163882 CNST consortin, connexin sorting... XM_011544110.3 84.7% 84.4% (many diffs)
5 human 163882 CNST consortin, connexin sorting... XM_011544111.1 84.7% 84.4% (many diffs)
6 human 163882 CNST consortin, connexin sorting... XM_017000487.1 67.7% 67.1% (many diffs)
7 human 163882 CNST consortin, connexin sorting... XM_011544112.1 66% 65.7% (many diffs)
8 human 163882 CNST consortin, connexin sorting... XM_005273083.5 62% 58.7% (many diffs)
9 human 163882 CNST consortin, connexin sorting... XM_011544113.2 62% 58.7% (many diffs)
10 human 163882 CNST consortin, connexin sorting... XM_011544114.2 60.8% 58.3% (many diffs)
11 human 163882 CNST consortin, connexin sorting... XM_017000488.1 60.8% 58.3% (many diffs)
12 human 163882 CNST consortin, connexin sorting... XM_017000489.2 60.8% 58.3% (many diffs)
13 human 163882 CNST consortin, connexin sorting... XM_024453697.1 56% 55.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1914
ORF length:
1848
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tgacagcgat actcctacat attatctgca aatagaacca caagatggat
121gtcatcctgg tgacagcgtg gaaaggagtg tgacctgtct gccttctgca tcagatgaaa
181atgaaaatca gcttgacggg gacgggcatg agcatctgac cagcagtgac agtgcgatgg
241gaaagcccca agtgtctgag caggacagtc tcaataataa tgaaagctgc acattgagct
301gcgaggtggc tgcaggtgag aactcgcaaa acaccctttg tgaagcctcc agagatgaac
361aggccttctt gggaaaggac aaaaaaattc ctggaaaaag aagtccaaga agcaaaaaag
421ggactgctaa gaagatacca ccaggacttt tttcaggaga tattgcacct ttaatgcaag
481aaaaagtact aagcgcagtc acatatgctg ttgatgatga agaagctgct gaagtaaatg
541ctaatgagca gccagaggcg ccaaagcttg ttctgcagtc tctgttttca cttatacgag
601gtgaagttga gcagttggat tcaagagcac ttcccctttg ccttcatcag atagcagaat
661cctatttcca ggaggaggac tatgagaaag caatgaaatt cattcagcta gaacgattgt
721atcatgagca attgctcgca aatctttctg ccattcaaga acagtgggaa acaaaatgga
781aaactgtgca accacataca gttacggctc taaggaattc agaaaaggga tttaatggtg
841aagattttga acggcttacg aaaatttgtg caacacatca agatcctctt ttatccaaac
901ataagatagc agctgtggag aagtcccagg aaaggaaatg ctccacgcag ttactagtgt
961ctgaagatcc aaaggaagga ggagctacca ccaaagagtc agagagtaaa acttgtctcg
1021gcacagagtc aagtaaagaa agccaacata cagtggagcc cctggggagc agtccctgct
1081gtcatcagat ggacgtgcaa acagattccc caagcctttc ggtaactgca ggaaaggacc
1141acatggagga gctgctctgc agcgctgaag ccacgttagc gctccacacc cagtcctccg
1201agacagcagg gagcccgtct gggccagact cttctgagga tgcttgtgag gatgacagtc
1261gcttgcagct ggctcaaaca gaggcctgcc aggatgtggc cagaatagag ggcattgctg
1321aagaccctaa ggtgtttctt tccagcaagt caaagacaga gccgttgatt tcaccaggct
1381gtgaccgtat acctcctgca ttgatttctg agggtaaata ttcacaggct cagaggaaag
1441aactccgttt gccacttcgg gatgcttctg aggcgttgcc cacagaccaa cttgagaaca
1501atgaattaaa tgagctgcag cagcctgatc ttacagacag tgatggaaaa tcaccacagg
1561cgcaggctga ctcagacggt tctgagaatg tgctctgtgg aaataatcaa atatctgact
1621taggcatact gcttccagag gtgtgtatgg ccccagagga aaagggagat aaagacgacc
1681aactcaacaa agaaacagaa gactatttga acagcctttt agaaggatgt ttaaaagata
1741ctgaagattc cctttcctat gaagataacc aagacgacga ctccgatctc cttcaagatc
1801tctctcctga agaagcatcc tatagtctcc aggagaatct gccttctgat gagagctgtc
1861tttctcttga tgatcttgcc aaaaggatag agattgcaga ggtaaatcag agatgcccaa
1921ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1981cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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