Construct: ORF ccsbBroad304_09758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014379.1_s304c1, BRDN0000411399
- Derived from:
- ccsbBroadEn_09758
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOBEC3H (164668)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09758
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 164668 | APOBEC3H | apolipoprotein B mRNA editi... | NM_001166002.3 | 100% | 100% | |
| 2 | human | 164668 | APOBEC3H | apolipoprotein B mRNA editi... | NM_181773.5 | 99.4% | 98.9% | 542_544delAGC |
| 3 | human | 164668 | APOBEC3H | apolipoprotein B mRNA editi... | NM_001166003.3 | 91% | 90.5% | 544_545delAT;549_600del |
| 4 | human | 164668 | APOBEC3H | apolipoprotein B mRNA editi... | XM_011529992.3 | 91% | 90.5% | 544_545delAT;549_600del |
| 5 | human | 164668 | APOBEC3H | apolipoprotein B mRNA editi... | XM_011529990.2 | 89.6% | 89.1% | 544_547delGGGC;551_609del |
| 6 | human | 164668 | APOBEC3H | apolipoprotein B mRNA editi... | XM_011529991.3 | 89.6% | 89.1% | 544_547delGGGC;551_609del |
| 7 | human | 164668 | APOBEC3H | apolipoprotein B mRNA editi... | NM_001166004.2 | 77.5% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 612
- ORF length:
- 546
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tctgttaaca gccgaaacat tccgcttaca gtttaacaac aagcgccgcc 121 tcagaaggcc ttactacccg aggaaggccc tcttgtgtta ccagctgacg ccgcagaatg 181 gctccacgcc cacgagaggc tactttgaaa acaagaaaaa gtgccatgca gaaatttgct 241 ttattaacga gatcaagtcc atgggactgg acgaaacgca gtgctaccaa gtcacctgtt 301 acctcacgtg gagcccctgc tcctcctgtg cctgggagct ggttgacttc atcaaggctc 361 acgaccatct gaacctgggc atcttcgcct cccgcctgta ctaccactgg tgcaagcccc 421 agcagaaggg gctgcggctt ctgtgtggat cccaggtccc ggtggaggtc atgggcttcc 481 cagagtttgc tgactgctgg gaaaactttg tggaccacga gaaaccgctt tccttcaacc 541 cctataagat gttagaggag ctagataaaa acagtcgagc cataaagcga cggcttgaga 601 ggataaagtc ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 661 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 721 caatcaacct ctggatta