Construct: ORF ccsbBroad304_09864
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016176.1_s304c1, BRDN0000411669
- Derived from:
- ccsbBroadEn_09864
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC39A12 (221074)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09864
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_001145195.2 | 99.9% | 100% | 99T>C |
| 2 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_001282733.1 | 99.8% | 99.8% | 99T>C;1420_1421insAGC |
| 3 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_152725.3 | 94.5% | 94.6% | 99T>C;1421_1422ins111 |
| 4 | human | 221074 | SLC39A12 | solute carrier family 39 me... | NM_001282734.1 | 80.6% | 80.6% | 0_1ins402 |
| 5 | mouse | 277468 | Slc39a12 | solute carrier family 39 (z... | XM_017318950.1 | 38.1% | 38.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2142
- ORF length:
- 2073
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgcttccgg acaaagctct cagtatcctg ggtgccattg tttcttctac 121 tcagccgtgt tttttctact gagacagaca aaccctcagc ccaggacagc agaagccgtg 181 ggagttcagg ccaaccggca gacctgctac aggttctctc tgctggtgac cacccacccc 241 acaaccactc aagaagcctc atcaaaacat tgttggagaa aactgggtgc ccacggagga 301 gaaacggaat gcaaggagat tgcaatctgt gctttgaacc agatgcacta ttactaatag 361 ctggaggaaa ttttgaagat cagcttagag aagaagtggt ccagagagtt tctcttctcc 421 ttctctatta cattattcat caggaagaga tctgttcttc aaagctcaac atgagtaata 481 aagagtataa attttaccta cacagcctac tgagcctcag gcaggatgaa gattcctctt 541 tcctttcaca gaatgagaca gaagatatct tggctttcac caggcagtac tttgacactt 601 ctcaaagcca gtgtatggaa accaaaacgc tgcagaaaaa atctggaata gtgagcagtg 661 aaggtgctaa tgaaagtacg cttcctcagt tggcagccat gatcattact ttgtccctcc 721 agggtgtttg tctgggacaa ggaaacttgc cttccccaga ctactttaca gaatatattt 781 tcagttcctt gaatcgtacg aataccctcc gcctatcaga actagaccaa ctcctcaaca 841 ctctctggac cagaagtact tgtatcaaaa atgagaaaat ccatcaattt caaaggaaac 901 aaaacaacat aataacccat gatcaggact attctaattt ctcttcatcc atggaaaaag 961 agtctgagga tggtccagtt tcctgggatc agacctgctt ctctgctagg cagctggtgg 1021 agatatttct acagaagggc ctctcactca tttctaagga ggactttaag caaatgagtc 1081 cagggatcat ccagcagctc ctcagctgct cctgccactt acccaaggac caacaagcaa 1141 agctgccacc taccactctg gagaaatacg gctacagcac ggtggctgtc acccttctca 1201 cactgggctc catgctgggg acagcgctgg tccttttcca tagctgtgag gagaactaca 1261 ggcttatctt acagctgttt gtgggcttgg ccgtcgggac actgtctggg gacgctctgc 1321 tccaccttat ccctcaggtt cttggtttac ataagcagga agccccagaa tttgggcatt 1381 tccatgaaag caaaggtcat atttggaaac tgatgggatt aattggaggc atccatggat 1441 ttttcttgat agaaaaatgt tttattcttc ttgtatcacc aaatgacaag cagggcctgt 1501 cattggttaa tgggcacgtg ggtcattccc accatcttgc actcaactct gaattaagtg 1561 accaggcagg cagaggcaaa tctgcttcaa ctatccagtt gaaaagccca gaagattcac 1621 aggcagctga aatgcctata ggcagtatga cagcctccaa cagaaaatgt aaagccatta 1681 gcttgttagc aatcatgatt ctggttgggg acagcctgca taattttgca gatggcctag 1741 ccataggagc agccttctca tcatcatccg agtcaggagt gaccactacg attgctatct 1801 tgtgtcatga aatcccacat gaaatgggag actttgccgt gctcttaagc tctggacttt 1861 ctatgaagac tgccatcctg atgaatttta taagctccct aactgccttc atgggattat 1921 acattggcct ttccgtgtca gctgatccat gtgttcaaga ctggatcttc acagtcactg 1981 ctgggatgtt cttatattta tccttggttg aaatgcttcc tgaaatgact catgttcaaa 2041 cacaacgacc ctggatgatg tttctcctgc aaaactttgg attgatccta ggttggcttt 2101 ctctcctgct cttggctata tatgagcaaa atattaaaat attgccaact ttcttgtaca 2161 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 2221 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta