Construct: ORF ccsbBroad304_09868

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010832.1_s304c1, BRDN0000406674
Derived from:
ccsbBroadEn_09868
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MICU2 (221154)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09868
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221154 MICU2 mitochondrial calcium uptake 2 NM_152726.3 99.7% 99.5% 30G>A;614G>A;779A>T
2 human 221154 MICU2 mitochondrial calcium uptake 2 XR_429214.4 62.4% (many diffs)
3 human 221154 MICU2 mitochondrial calcium uptake 2 XM_017020433.1 57.9% 57.6% 0_1ins546;68G>A;233A>T
4 human 221154 MICU2 mitochondrial calcium uptake 2 XR_001749501.2 53.1% (many diffs)
5 mouse 68514 Micu2 mitochondrial calcium uptake 2 NM_028643.3 83.1% 84.7% (many diffs)
6 mouse 68514 Micu2 mitochondrial calcium uptake 2 XM_006519484.3 83% 84.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1368
ORF length:
1302
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggcggctgcg ggtagctgcg cgcgagtggc ggcctggggc ggaaaactgc
121gacgggggct cgctgtcagc cgacaggctg tgcggagtcc cggccccttg gcagcggcag
181tggccggcgc ggccctggca ggagcaggag cggcctggca ccacagccgc gtcagtgttg
241cggcgcggga tggcagtttt acagtctccg cacagaaaaa tgttgaacat ggaataatat
301atattgggaa accgtctctt cgtaagcagc gcttcatgca gttttcttca ctcgaacatg
361aaggagaata ttatatgaca ccacgagact tcctcttctc agtgatgttt gagcaaatgg
421aacgtaaaac ttcagtcaag aagctgacaa aaaaggacat cgaggataca ctgtcaggga
481tccaaacagc tggctgtgga tcaacttttt tcagagacct tggcgataaa gggctaattt
541catataccga gtatcttttc ttgcttacaa tcctcactaa accccattct ggatttcatg
601ttgcttttaa aatgctggat acagatggta atgagatgat tgaaaaaagg gaatttttta
661agctgcagaa gatcataaat aaacaagatg acttgatgac agtgaaaact aatgaaactg
721gatatcagga agcaatagtg aaagaacctg aaattaacac aactcttcag atgcgtttct
781ttggaaaaag aggacaaaga aaacttcatt ataaagaatt tcgaagattt atggaaaatt
841tactaacaga gattcaagaa atggaattcc ttcagttttc taaaggtttg agtttcatga
901gaaaagaaga ctttgcagag tggctacttt ttttcactaa cactgaaaat aaagatattt
961attggaaaaa tgtgagagag aagttgtcag caggagagag cattagtttg gatgaattca
1021agtcattttg ccattttaca acccacttgg aagactttgc tattgccatg cagatgttca
1081gtttagctca tcgtcctgtc agactagcgg agtttaagag agctgtgaaa gtagcaacag
1141gacaagaact ctcaaacaat attttggaca ctgtctttaa gatctttgat ttggatggtg
1201atgaatgtct tagtcatgaa gagtttcttg gggtgttaaa aaacagaatg catcgaggtt
1261tatgggtacc acaacatcag agtatacaag aatactggaa gtgtgtgaag aaagaaagca
1321ttaaaggagt aaaagaagtc tggaaacaag ctggaaaagg tcttttttac ccaactttct
1381tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1441aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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