Construct: ORF ccsbBroad304_09900

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017542.1_s304c1, BRDN0000399951
Derived from:
ccsbBroadEn_09900
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCM9 (254394)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09900
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 254394 MCM9 minichromosome maintenance ... NM_153255.4 99.9% 100% 573G>A
2 human 254394 MCM9 minichromosome maintenance ... NM_017696.2 34% 33.6% (many diffs)
3 mouse 71567 Mcm9 minichromosome maintenance ... XM_006512856.3 86.1% 88.9% (many diffs)
4 mouse 71567 Mcm9 minichromosome maintenance ... XM_006512857.3 85.9% 89.7% (many diffs)
5 mouse 71567 Mcm9 minichromosome maintenance ... XM_017314097.1 30% 31.1% (many diffs)
6 mouse 71567 Mcm9 minichromosome maintenance ... NM_027830.2 26.3% 27.3% (many diffs)
7 mouse 71567 Mcm9 minichromosome maintenance ... XR_380074.3 19.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1239
ORF length:
1173
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tagcgatcaa gttacactgg ttggtcaagt gtttgagtca tatgtttcgg
121aataccataa gaatgatatt cttctaatct tgaaggaaag ggatgaagat gctcattacc
181cagttgtggt taatgccatg actctgtttg agaccaacat ggaaatcggg gaatatttca
241acatgttccc cagtgaagtg cttacaattt ttgatagtgc actgcgaagg tcagccttga
301caattctcca gtccctttct cagcctgagg ctgtttccat gaaacagaat cttcatgcca
361ggatatcagg tttgcctgtc tgtcctgagc tggtgaggga acacatacct aaaaccaagg
421atgtgggaca ctttttatct gtcactggga cagtgattcg aacaagtctg gtgaaggttc
481tggagtttga gcgggattac atgtgtaaca aatgcaagca tgtgtttgtg atcaaggctg
541actttgagca gtattacacc ttttgccggc catcctcgtg tcccagcttg gagagctgtg
601attcctctaa attcacttgc ctctcaggct tgtcttcatc tccaaccagg tgtagagatt
661accaggaaat caaaattcag gaacaggttc aaaggctatc tgttggaagt attccacgat
721ctatgaaggt tattctggaa gatgacttag tggatagttg caaatctggt gatgacctca
781ctatttacgg gattgtaatg caacggtgga agccctttca gcaagatgtg cgctgtgaag
841tggagatagt cctgaaagca aattacatcc aagtaaataa tgagcagtcc tcagggatca
901tcatggatga ggaggtccaa aaggaattcg aagatttttg ggaatactat aagagcgatc
961cctttgcagg aaggaatgta atattggcta gcttgtgccc tcaagtgttt ggaatgtatc
1021tagtaaagct tgctgtggcc atggtgctgg ctggtgggat tcaaaggact gatgctacag
1081gaacacgggt cagaggagaa tctcatcttt tattggttgg ggatcctggc acagggaaat
1141ctcagttcct caaatatgca gcaaagatta caccaagatc tgtgctgacc acaggaattg
1201gatctactag tgcaggtatt gtatgtgaca atttcaagta cccaactttc ttgtacaaag
1261tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1321gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)