Construct: ORF ccsbBroad304_09928
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018145.1_s304c1, BRDN0000401555
- Derived from:
- ccsbBroadEn_09928
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC14L3 (266629)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09928
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 266629 | SEC14L3 | SEC14 like lipid binding 3 | NM_174975.4 | 99.8% | 99.7% | 930T>C;1005T>G |
| 2 | human | 266629 | SEC14L3 | SEC14 like lipid binding 3 | XM_011530128.2 | 90.5% | 86.5% | (many diffs) |
| 3 | human | 266629 | SEC14L3 | SEC14 like lipid binding 3 | NM_001257379.1 | 85% | 85% | 0_1ins177;753T>C;828T>G |
| 4 | human | 266629 | SEC14L3 | SEC14 like lipid binding 3 | NM_001257382.1 | 85% | 85% | 0_1ins177;753T>C;828T>G |
| 5 | human | 266629 | SEC14L3 | SEC14 like lipid binding 3 | NM_001257378.1 | 80.4% | 80.2% | (many diffs) |
| 6 | mouse | 380683 | Sec14l3 | SEC14-like lipid binding 3 | NM_001029937.2 | 90.6% | 97% | (many diffs) |
| 7 | mouse | 380683 | Sec14l3 | SEC14-like lipid binding 3 | XM_011243730.2 | 78.3% | 83.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1269
- ORF length:
- 1200
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcggccga gttggagacc tgagccccaa acaggcagag accctggcca 121 agttccgaga aaacgtccag gatgtgcttc ctgccctgcc caaccctgat gattatttcc 181 ttctacgctg gctccgagct cggaattttg acttgcagaa gtcggaggct ttgctccgca 241 agtacatgga gttccggaag accatggata ttgaccatat ccttgattgg cagcccccag 301 aggtgatcca gaagtacatg cctgggggcc tgtgtggcta tgaccgtgat ggctgccccg 361 tgtggtatga catcattggg ccacttgatc ccaaggggtt gctcttctca gtcaccaagc 421 aggacctgct caagaccaag atgagggact gtgagcgcat cctgcatgag tgtgacctgc 481 agacagagag gctagggaag aagattgaga ccatcgtgat gatatttgac tgtgagggcc 541 tgggactgaa acacttctgg aaacctctgg tagaagtgta ccaggagttc tttggcctcc 601 ttgaagagaa ttacccagag accctgaagt tcatgctcat cgtgaaagct accaaactgt 661 tccctgtggg ctacaacctc atgaagccat tcctgagtga ggacactcgc aggaaaatta 721 ttgtgttggg aaataactgg aaggaaggtt tgctgaaact catcagtcct gaggaactgc 781 ctgcccagtt tgggggcacc ctgactgacc cagatgggaa ccccaaatgt ttaaccaaga 841 ttaactatgg cggggagatc cccaagtcca tgtacgtgcg ggaccaggtg aagactcagt 901 acgagcactc ggtgcagatc aaccgcggct catcacacca agtggaatac gagatcctat 961 ttccaggctg cgttctcagg tggcagttct catctgacgg tgcggacatc ggcttcggag 1021 ttttcctgaa gaccaagatg ggggagcgac agcgggcagg ggagatgaca gaggttctac 1081 ccagccagcg ctataacgcc cacatggtgc ccgaggatgg gaacctcacc tgctcagagg 1141 ccggcgtcta tgtcctacgc ttcgacaaca cctatagctt tgtccacgcc aagaaggtca 1201 gcttcacagt ggaggtcctg ctccctgacg agggcatgca gaaatatgat aaggagctca 1261 cccctgtctt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1321 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1381 tcaacctctg gatta