Construct: ORF ccsbBroad304_10035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008304.1_s304c1, BRDN0000401958
- Derived from:
- ccsbBroadEn_10035
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZKSCAN2 (342357)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10035
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 342357 | ZKSCAN2 | zinc finger with KRAB and S... | NM_001012981.5 | 99.9% | 99.8% | 81T>C;1845A>T |
| 2 | human | 342357 | ZKSCAN2 | zinc finger with KRAB and S... | XM_017023200.2 | 78.1% | 76.8% | 0_1ins623;53_63del;1233A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2970
- ORF length:
- 2901
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgtcgcc ctcgactctc agatcgacgc gcccctggag gttgagggat 121 gcctaataat gaaggtggaa aaggaccccg agtgggcatc agagcccatt ctggaaggat 181 cggatagctc tgagaccttc cgcaaatgct tcaggcaatt ctgttatgag gatgtgactg 241 gaccccatga agctttcagt aaactctggg aactttgctg ccggtggctg aagccagaaa 301 tgcgttccaa ggagcaaata cttgagctgc tggtgattga gcagtttctc accattttac 361 ccgagaagat tcaggcttgg gcacagaagc agtgtccgca aagtggagag gaagcggtgg 421 ccctggtagt gcatttggag aaagagactg gaagactaag acagcaggtc agcagtcccg 481 tgcaccggga gaagcactcc ccacttggag cagcgtggga ggtggcagac ttccagccag 541 agcaggtgga gacccaaccc agggcggtgt ctcgggagga acctggaagc ctccactcag 601 gacaccagga acagctgaac cgaaagcgag aacgtcggcc cttacccaag aatgctcggc 661 cttctccctg ggttcctgcc cttgctgatg aatggaatac cctagatcag gaagtgacaa 721 ccacacggct tcctgctggg tcccaggaac cagtgaaaga tgtccacgtg gccagaggct 781 tttcctacag aaagagtgtg catcagattc ctgcccaaag ggacctctac cgggatttca 841 ggaaggagaa tgttgggaac gtggtctccc tgggaagtgc agtgtctaca tctaacaaga 901 taacccggtt ggaacagaga aaggagccat ggactctagg tctgcattcc tctaacaaga 961 gaagtatcct acgaagcaac tacgtcaagg aaaagtcagt tcatgctatt caggtccctg 1021 caaggagtgc aggaaaaaca tggagagagc agcagcagtg gggtttagaa gatgaaaaga 1081 tagcaggtgt gcattggagc tatgaggaaa caaagacttt cctggcaatt ctcaaagagt 1141 ctcgctttta tgaaacactt caggcctgtc cccgaaatag ccaagtgtat ggtgctgtgg 1201 ctgaatggtt gcgagaatgt ggcttcctta gaaccccaga acagtgtcga accaagttca 1261 aaagtctcca gaaaagctat cgaaaggtga gaaatggcca catgctagaa ccctgcgcct 1321 tctttgagga catggatgct ttgttgaacc ctgcagcccg tgctccgtcc actgataaac 1381 caaaggagat gatacctgtc cccagactga agagaattgc catcagtgct aaggaacaca 1441 tcagcttggt ggaggaggag gaagctgcag aagattctga tgatgatgaa ataggcatcg 1501 aatttatccg caagtctgaa atccatggtg cccctgtctt gtttcagaat ctcagtggcg 1561 tgcactgggg ctatgaagaa accaagactt ttcttgatat cctccgtgag actcggtttt 1621 atgaagcgct tcaagcctgt catcggaaga gcaaattgta tggggctgta gctgaacagc 1681 ttcgagagtg cggcttcctc cggacaccag aacagtgccg aaccaagttc aaaagccttc 1741 agaagagtta ccgcaaggtg aaaaatggcc acgtgctaga gtcctgcgcg ttctacaagg 1801 agatggatgc cctgattaac tctcgggcat ctgctccttc ccccagcacc ccagaggaag 1861 tcccatcacc ttcaaggcaa gaaagagggg gtattgaggt tgaaccccag gatcctacag 1921 gctgggaacc tgaagagacc tcacaggagg cagtaataga agactcttgc agtgagagaa 1981 tgagcgagga ggaaattgtg caagagccag agttccaggg acctccaggt ctactgcaga 2041 gcccaaatga ttttgaaatc ggaagtagca tcaaggagga tccaacacag atagtatata 2101 aggacatgga acagcatagg gcattaatag aaaagtctaa aagagttgtt tcccagagta 2161 ccgaccccag caaatatcgc aaaagggaat gcatctcagg aagacaatgg gaaaatcttc 2221 aaggaattag acagggaaag ccgatgtctc agcctagaga tttagggaaa gccgttgtgc 2281 atcagaggcc ttttgtgggg aagagaccct acagacttct caaatatgga gaaagctttg 2341 gaaggagcac tcgtctgatg tgccggatga cccaccacaa ggagaatcct tacaagtgtg 2401 gtgtctgtgg gaagtgcttt ggtagaagca ggagcctgat cagacaccaa agaatccaca 2461 caggcgaaaa accttttaaa tgtcttgact gtggaaaaag ctttaatgac tcctcaaatt 2521 ttggtgccca ccagagaatc cacacaggag agaaacccta cagatgcgga gagtgtggaa 2581 aatgctttag tcagagctct agtcttatta tacatcagag aacgcacacc ggtgagaagc 2641 cctatcagtg tggagagtgt gggaaaagtt tcaccaacag ttctcatttc agcgcccacc 2701 ggagagttca cactggggag aatccctaca aatgtgtgga ctgtgaaaaa agtttcaata 2761 actgtacgag atttcgagaa catcggagaa tacacactgg agagaagccc tatggatgtg 2821 cccagtgtgg caaacgtttc agtaagagtt ctgttcttac caaacatcgg gaagttcatg 2881 tgagagaaaa gcctctgcca caccctccat ctctgtattg ccctgagaac ccacataagg 2941 gaaagactga tgaatttagg aaaacttttt tgccaacttt cttgtacaaa gtggttggta 3001 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 3061 ggtggcgcgt taagtcgaca atcaacctct ggatta