Construct: ORF ccsbBroad304_10127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016516.1_s304c1, BRDN0000462163
- Derived from:
- ccsbBroadEn_10127
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTHD1 (401124)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10127
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401124 | DTHD1 | death domain containing 1 | NM_001170700.3 | 86.1% | 85.9% | 1_375del;911T>A;2029C>T |
| 2 | human | 401124 | DTHD1 | death domain containing 1 | XM_011513693.2 | 85.2% | 85.1% | (many diffs) |
| 3 | human | 401124 | DTHD1 | death domain containing 1 | XM_011513694.2 | 82.9% | 82.8% | (many diffs) |
| 4 | human | 401124 | DTHD1 | death domain containing 1 | XM_017008191.1 | 82.7% | 73.4% | (many diffs) |
| 5 | human | 401124 | DTHD1 | death domain containing 1 | XM_011513695.2 | 80.1% | 80% | (many diffs) |
| 6 | human | 401124 | DTHD1 | death domain containing 1 | NM_001136536.5 | 78.1% | 78.1% | (many diffs) |
| 7 | human | 401124 | DTHD1 | death domain containing 1 | XM_011513696.2 | 77.2% | 77.2% | (many diffs) |
| 8 | human | 401124 | DTHD1 | death domain containing 1 | XM_017008192.1 | 74.7% | 72.8% | (many diffs) |
| 9 | human | 401124 | DTHD1 | death domain containing 1 | XM_017008193.1 | 46.9% | 46.5% | (many diffs) |
| 10 | human | 401124 | DTHD1 | death domain containing 1 | NR_160267.1 | 35.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2412
- ORF length:
- 2343
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcatgatgaa tgtactccac agcagacaat gtcctccatt caagatacca 121 aagcagcaga cattgctgca agaggggaac taaatgtcat agaaacagct actgtttctc 181 ccacaaatgg agaggaaagt cattacacaa accaggtcca gttagaaaaa aataaaacac 241 atatgagttc agcattagtg gaaaaagaaa acaatacatc actgaatgga cgtgtactgg 301 ggcaagaaga gtcacagaat aaaatgttcc cagataatgc agaaaatgaa gatgataaac 361 aaatagaaca catgactgtt gagaacataa atggcaacag ggaagagact catggcataa 421 ttcagacaac agagacagaa attcaagaga cttcagaaag cccaagagaa gagatgacca 481 catcctcaat aatatgtgat atctccaaga aatatataaa tagtactctt cccaatgatt 541 cagagaatat aaagcacaag aataacataa tggaaaagga gtatcttgat gtgctgagtg 601 atgatactgg cccccaagtg tcttgttata ttacagcacc atcatatgtt ctacaacaac 661 tagaatgccg gataataaat cacatgagtt ctttaatagt gggtgataat gaagagttag 721 ttagcaacgt cataactatt gaatgctcag ataaggaaaa gagagttcca tttccaatag 781 gcattgcaat tccatttact gcacgttaca gaggaaatta cagagatatc atggtgaaag 841 tgtgtgacat aaaccttcaa tcaagttacc taaacccaaa ttcactagaa ggaatgaagg 901 gaggttataa ggggacctgt gcttcagtaa aagtttacaa attgggtatc ttttctgttg 961 tgtcttgttt aaagaaagag tcgttcacag taacaaagaa aggcctcgct cttaagtcaa 1021 gcatggattc ccgaatatcc ttaaattacc ctccaggagt ttttacctct ccagtgctgg 1081 tgcagttaaa gatccaacca gttgacccag ctctggtggc acatttaaaa gcacagcaag 1141 atactttcta ctcagtccaa tccacaagcc ctctgattca cattcagcac ccatcaactt 1201 atccttttca gaagccagtc actttgtttt taccttgttc tccatacctt gataaaaaca 1261 accttggttc tgagatagat cataaaagaa gagcaagtgc cacaataaat aggattacac 1321 cttcgtattt caaccggaca aaaattgcct ccataagaaa acctaggaaa aatgccagtg 1381 aatgtttgaa attactggga ttcagaagcc aagacagtgg ttggtgtggg cttgatgatg 1441 ttgtgaaaac catacagagc ggcttggtat cagttgaatt gtatgaacat ttggagaggt 1501 ttattgtact tcacctctct tccaccatgg acaatagtca tttggttact tttgtgaaat 1561 ctttagagga agccatgctc agcaccactg cctgcatagt actgtctcac cagaaggaca 1621 atccacatag aatagctgtt ttagtggtgc cttccaaaga tttaagccag gtgcttaagg 1681 acctgcactt ggaagggttt ggaggacctc cagagccatc ttgtcatttc caagttcgag 1741 aaggagaaca acttctttta agatttactg gaaacatatt tgcttcaagc aacgggaagg 1801 attatggaaa agactacaca cttatttttc acttgcaaag aaaacctagg ctggaactcc 1861 aaatcaaaga agtggacgaa tttggaaact atagttgccc tcattacaaa ggcaccattg 1921 tcgtttataa agtacctaaa ggaaagatag tccccaactt gaatcaatct ctcgtaatta 1981 atgaaaacca ttctcagttg ccaatttgca aattaccatt gaaattgcca aagcataaga 2041 aattaatcaa ccgtccacag agtaccaaaa gagtttctaa ggatcctgta gaagcccttt 2101 gggataactt gctccattgg ctggctgagg agctctcaga agaaaatgct gagtctcttt 2161 cctcaactct ccctctgcgc cgtagcacca ttcagctcat caaactcaag aaccctgatg 2221 atctcacaga acagatccac gagtttcttt gcttctggaa aaaatcgctt ccaactttca 2281 ccgacaaact tcgcctcctg gctcgacatc tccgcaagat tggcaggagt gatcttgcag 2341 aagagctcaa attcaagtgg gaaaataaag tgttcactga accacagcag tgttttgatg 2401 tagcccctga gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2461 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2521 caatcaacct ctggatta