Construct: ORF ccsbBroad304_10131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016697.1_s304c1, BRDN0000461116
- Derived from:
- ccsbBroadEn_10131
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RAB19 (401409)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10131
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401409 | RAB19 | RAB19, member RAS oncogene ... | NM_001008749.3 | 99.6% | 5% | 12C>G;34G>T |
| 2 | human | 401409 | RAB19 | RAB19, member RAS oncogene ... | XM_005249996.3 | 99.6% | 5% | 12C>G;34G>T |
| 3 | human | 401409 | RAB19 | RAB19, member RAS oncogene ... | XM_017012207.1 | 99.6% | 5% | 12C>G;34G>T |
| 4 | human | 401409 | RAB19 | RAB19, member RAS oncogene ... | XM_005249997.3 | 94.1% | 5.3% | 12C>G;34G>T;165_166ins36 |
| 5 | human | 401409 | RAB19 | RAB19, member RAS oncogene ... | XM_005249998.3 | 93.2% | 5.4% | 12C>G;34G>T;165_166ins42 |
| 6 | mouse | 19331 | Rab19 | RAB19, member RAS oncogene ... | NM_011226.1 | 86% | 3.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 102
- ORF length:
- 33
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcacttctcg agctcagcca gggcagcaga ttagaacttt gactatttgt 121 tcaagattat cctcattggg gattccaatg tggggaagac gtgtgtggtg cagcatttca 181 agtctggagt ctacactgag acacagcaga acacgattgg agtggacttt accgtgcgtt 241 cccttgatat tgacggcaaa aaagtgaaga tgcaggtgtg ggacacagct ggccaggagc 301 gcttccgcac catcacccaa agctactacc gcagtgccca cgcagccatc atcgcctatg 361 acctcacccg gcggtccacg ttcgagtcca tccctcactg gattcatgag atagagaaat 421 atggagctgc aaatgtggtc attatgctga ttggaaataa atgtgacctc tgggaaaagc 481 ggcacgtcct gttcgaggat gcctgcacac tggctgagaa gtacggcctc ctggccgttt 541 tggagacatc tgccaaggag tcaaagaaca tagaagaagt cttcgtgctc atggccaagg 601 agctgatcgc gcgcaacagc ctgcacctat atggggagag tgccctgaac ggcctccccc 661 tggactccag ccccgttctt atggcccagg gtccaagtga aaagacccac tgcacttgct 721 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 781 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 841 ggatta