Construct: ORF ccsbBroad304_10240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015339.1_s304c1, BRDN0000404550
- Derived from:
- ccsbBroadEn_10240
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CROT (54677)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10240
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54677 | CROT | carnitine O-octanoyltransfe... | NM_001243745.2 | 100% | 100% | |
| 2 | human | 54677 | CROT | carnitine O-octanoyltransfe... | XM_011516337.3 | 14.6% | 13.7% | (many diffs) |
| 3 | human | 54677 | CROT | carnitine O-octanoyltransfe... | NM_021151.4 | 13.9% | 13.1% | (many diffs) |
| 4 | human | 54677 | CROT | carnitine O-octanoyltransfe... | NM_001143935.2 | 13.3% | 12.5% | (many diffs) |
| 5 | mouse | 74114 | Crot | carnitine O-octanoyltransfe... | NM_023733.3 | 12.4% | 11.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 327
- ORF length:
- 261
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aaatcaattg gctaaatcaa ctgaagaacg aacatttcag taccaggatt 121 ctcttccatc actgcctgtt ccttcacttg aagaatcatt aaaaaaatac cttgaatcag 181 tgaaaccatt tgcaaatcaa gaagaatata agaaaactga agaaatagtt caaaaatttc 241 aaagtgggat tggagaaaaa ttgcaccaga aattgcttga aagagcaaaa ggaaaaagaa 301 attgggtatt tgttgttata attgaatacc caactttctt gtacaaagtg gttggtaagc 361 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 421 ggcgcgttaa gtcgacaatc aacctctgga tta