Construct: ORF ccsbBroad304_10260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003782.1_s304c1, BRDN0000404477
- Derived from:
- ccsbBroadEn_10260
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF170 (81790)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10260
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81790 | RNF170 | ring finger protein 170 | NM_001160224.2 | 56.9% | 54.2% | (many diffs) |
| 2 | human | 81790 | RNF170 | ring finger protein 170 | NM_001160223.1 | 44.9% | 42% | 322_395del;423_774del |
| 3 | human | 81790 | RNF170 | ring finger protein 170 | NM_030954.4 | 44.9% | 42% | 322_395del;423_774del |
| 4 | human | 81790 | RNF170 | ring finger protein 170 | XM_006716404.2 | 44.9% | 42% | 322_395del;423_774del |
| 5 | human | 81790 | RNF170 | ring finger protein 170 | XM_011544666.3 | 44.9% | 42% | 322_395del;423_774del |
| 6 | human | 81790 | RNF170 | ring finger protein 170 | XM_017013881.1 | 44.9% | 42% | 322_395del;423_774del |
| 7 | human | 81790 | RNF170 | ring finger protein 170 | XM_006716405.3 | 38.8% | 28.3% | 134_135ins76;273_624del |
| 8 | human | 81790 | RNF170 | ring finger protein 170 | XR_001745605.1 | 9.6% | 1_56del;405_3619del | |
| 9 | human | 81790 | RNF170 | ring finger protein 170 | NR_027669.2 | 9.4% | 1_129del;478_3690del | |
| 10 | human | 81790 | RNF170 | ring finger protein 170 | NR_027668.2 | 8.8% | 1_359del;708_3920del | |
| 11 | human | 81790 | RNF170 | ring finger protein 170 | XR_001745604.2 | 8.7% | 1_247del;570_720del;747_3961del | |
| 12 | human | 81790 | RNF170 | ring finger protein 170 | XR_002956648.1 | 8.4% | 1_409del;732_882del;909_4123del | |
| 13 | human | 81790 | RNF170 | ring finger protein 170 | XR_001745606.1 | 7.2% | (many diffs) | |
| 14 | human | 81790 | RNF170 | ring finger protein 170 | XR_002956649.1 | 6.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc caaatatcaa ggtgaagttc aaagtttgaa actggatgat gattcagtta 121 tagaaggagt aagcgaccaa gtacttgtgg cagttgtggt cagtttcgct ttgattgcta 181 ccctggtata tgcacttttc agaaatgtac atcaaaacat tcacccagaa aaccaggagc 241 tagtaagggt acttcgagaa cagcttcaaa cagaacagga tgcacctgct gccactcgac 301 agcagttcta cactgacatg tactgtccca tctgcctgca ccaagcctcc ttcccggtgg 361 agaccaactg tggacatctt ttttgtggta accttactcc taacagtatt tggtgcccaa 421 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 481 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta