Construct: ORF ccsbBroad304_10267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000324.1_s304c1, BRDN0000407320
- Derived from:
- ccsbBroadEn_10267
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DSCR8 (84677)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10267
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026842.1 | 23.5% | 1_171del;283_471del | |
| 2 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026841.1 | 19.5% | 1_171del;252_348del;380_568del | |
| 3 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026840.1 | 18.9% | 1_171del;252_366del;398_586del | |
| 4 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026839.1 | 14.2% | (many diffs) | |
| 5 | human | 84677 | DSCR8 | Down syndrome critical regi... | NR_026838.1 | 12.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 177
- ORF length:
- 111
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggagcctgga cccaactttg ttactgtgag aaagggtctt cattcattca 121 agatggcatt tgttaagcac ctactacaaa ccttggaaat caagaaagtt ctggaatgcc 181 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 241 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 301 tta