Construct: ORF ccsbBroad304_10274

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017901.1_s304c1, BRDN0000408855
Derived from:
ccsbBroadEn_10274
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TBRG1 (84897)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10274
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84897 TBRG1 transforming growth factor ... NM_032811.3 33.8% 31.4% 1_63del;453_589del;618_1233del
2 human 84897 TBRG1 transforming growth factor ... XM_017018442.2 15.8% 3.6% 0_1ins253;165_780del
3 human 84897 TBRG1 transforming growth factor ... NR_016021.2 8.3% 1_283del;701_5007del
4 human 84897 TBRG1 transforming growth factor ... XR_428983.4 8% 1_287del;677_872del;901_5205del
5 human 84897 TBRG1 transforming growth factor ... XR_001748012.2 7.7% (many diffs)
6 human 84897 TBRG1 transforming growth factor ... XR_001748011.2 7.6% (many diffs)
7 mouse 21376 Tbrg1 transforming growth factor ... NM_025289.3 30% 27.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
486
ORF length:
417
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaaaaagctc ccgaagaaga gccagaatga gaagtaccgg ctgaagtacc
121tgcggctgcg caaagcggcc aaggccacgg tgtttgaaaa tgctgctatt tgtgatgaaa
181ttgctcgtct tgaggaaaaa tttcttaaag caaaagaaga aagaaggtac ttgctaaaga
241agctcctcca gcttcaggct ctaactgaag gggaagtaca ggctgcagct ccttcccaca
301gttccagttt gcccctgact tatggtgtgg ccagctctgt gggaactata cagggagctg
361ggcctatttc agggcccagc actggggctg aggaaccatt tgggaagaaa actaagaagg
421agaaaaaaga aaaaggcaaa gagaacaaca aactggaaga tcatcaccga ccgacctggc
481tttcattgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
541gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
601acctctggat ta

Download FASTA (ORF) (Full)