Transcript: Human XM_017018442.2

PREDICTED: Homo sapiens transforming growth factor beta regulator 1 (TBRG1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TBRG1 (84897)
Length:
7375
CDS:
2907..3689

Additional Resources:

NCBI RefSeq record:
XM_017018442.2
NBCI Gene record:
TBRG1 (84897)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018442.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000373593 CTATTGCAGTACTCGAATATA pLKO_005 3098 CDS 100% 15.000 21.000 N TBRG1 n/a
2 TRCN0000373592 ATCTTGTAATCGGGATGTAAT pLKO_005 3903 3UTR 100% 13.200 18.480 N TBRG1 n/a
3 TRCN0000307585 CAATTACCAGTGGGTGAAATT pLKO_005 3398 CDS 100% 13.200 9.240 N Tbrg1 n/a
4 TRCN0000016914 CCAGACCAGAAGTGTCTATAT pLKO.1 3135 CDS 100% 13.200 9.240 N TBRG1 n/a
5 TRCN0000373594 TACAGGGAGCTGGGCCTATTT pLKO_005 2934 CDS 100% 13.200 9.240 N TBRG1 n/a
6 TRCN0000016917 GAGGAACCATTTGGGAAGAAA pLKO.1 2975 CDS 100% 5.625 3.938 N TBRG1 n/a
7 TRCN0000016915 GAGAACAACAAACTGGAAGAT pLKO.1 3026 CDS 100% 4.950 3.465 N TBRG1 n/a
8 TRCN0000016916 GCAGATGCTTGTCATGCAGAA pLKO.1 3243 CDS 100% 4.050 2.835 N TBRG1 n/a
9 TRCN0000016913 CCCATGTACCTGACACATGAA pLKO.1 3597 CDS 100% 0.495 0.347 N TBRG1 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 5533 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 5534 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018442.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12875 pDONR223 100% 71.9% 71.9% None 1_219del n/a
2 ccsbBroad304_12875 pLX_304 0% 71.9% 71.9% V5 1_219del n/a
3 ccsbBroadEn_10274 pDONR223 100% 15.8% 3.6% None 0_1ins253;165_780del n/a
4 ccsbBroad304_10274 pLX_304 0% 15.8% 3.6% V5 0_1ins253;165_780del n/a
5 TRCN0000465708 ACATTACCATATTGCCCGAATTGG pLX_317 85.4% 15.8% 3.6% V5 0_1ins253;165_780del n/a
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