Construct: ORF ccsbBroad304_10284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002780.1_s304c1, BRDN0000406361
- Derived from:
- ccsbBroadEn_10284
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPLP0P2 (113157)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10284
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6175 | RPLP0 | ribosomal protein lateral s... | NM_001002.4 | 93.2% | 87.1% | (many diffs) |
| 2 | human | 6175 | RPLP0 | ribosomal protein lateral s... | NM_053275.3 | 93.2% | 87.1% | (many diffs) |
| 3 | human | 113157 | RPLP0P2 | ribosomal protein lateral s... | NR_002775.2 | 27% | 1_755del;1710_3525del | |
| 4 | mouse | 11837 | Rplp0 | ribosomal protein, large, P0 | NM_007475.5 | 86.4% | 84.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc cagggaagac agggtgacct ggaaacccaa ctacttcctt aagatcatcc 121 agctattgga tgattatctg aaatgtttca tcgtgggagc agacaatgtg aactccaagc 181 agatgcagca gatccgcatg tccctctgcg gcaaggccgt ggtgctgatg ggcaagaacg 241 ccatgacgcg caaggccatc cgagggcacc tggaaaacag cccagctctg gagaaactgc 301 tgcctcgcat ctgggagaat gtgggctttg tgttcaccaa ggaggacctc actgagatca 361 gggacctgct gctggccaat aaggtgccag ctgccacccg tgctggtgcc attaccccat 421 gtgaagtcac tgtgccagcc cagaatactg gtctggaccc cgagaagacc tcctttttcc 481 aggctttagg tatcatcact aaaatctcca ggggcgccat tgaaatcctg agtgatgcgc 541 agctgatcaa gacgggagac aaagtgggag ccagtgaagc cacgctgctg accatgctca 601 acatctcccc cttctcctct gggctggtca tccagcaggt gttcgacaac ggcagcatct 661 acaaccctga agtgcttgac atcacagagg aaactctgta ttctcgcttc ctggagggtg 721 tccgcaatgt tgccggtgtc tgtccgcaga tccactaccc aactgttgca tcagtgcccc 781 attctatcat cgacgggtac aaacgagtcc tggccttgtc tgtggagact gatgacaact 841 tcctgcttgc tgaaaaggtc aaggccttct tggctgatcc atctgccttt gtggctgcag 901 cccctgtggc tgctgccacc acagctgctc ctgctgctgc tgctgcagcc ccagctaagg 961 atgaagccaa ggaagagtcg gagggaggag tcagacgagg atatgggatt gggtctcttt 1021 gccaaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1081 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1141 ggatta