Construct: ORF ccsbBroad304_10332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012405.1_s304c1, BRDN0000398837
- Derived from:
- ccsbBroadEn_10332
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GK5 (256356)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10332
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 256356 | GK5 | glycerol kinase 5 | XR_002959504.1 | 58.9% | (many diffs) | |
| 2 | human | 256356 | GK5 | glycerol kinase 5 | XM_017006088.1 | 58.2% | 55.9% | (many diffs) |
| 3 | human | 256356 | GK5 | glycerol kinase 5 | NM_001039547.3 | 54.3% | 52.2% | (many diffs) |
| 4 | human | 256356 | GK5 | glycerol kinase 5 | XM_011512624.3 | 38.4% | 36.3% | (many diffs) |
| 5 | human | 256356 | GK5 | glycerol kinase 5 | XM_017006089.2 | 28.8% | 26.5% | (many diffs) |
| 6 | human | 256356 | GK5 | glycerol kinase 5 | XM_024453436.1 | 28.8% | 26.5% | (many diffs) |
| 7 | human | 256356 | GK5 | glycerol kinase 5 | NR_033289.2 | 8.2% | 1_130del;1034_10898del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 969
- ORF length:
- 903
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggggctgctc acggacccgg agcagagagc gcaggagccg cggtaccccg 121 gcttcgtgct ggggctggat gtgggcagtt ctgtgatccg ctgccacgtc tatgaccggg 181 cggcgcgggt ctgcggctcc agcgtgcaga aggtagaaaa tctttatcct caaattggct 241 gggtagaaat tgatcctgat gttctttgga ttcaatttgt tgccgtaata aaagaagcag 301 tcaaagctgc aggaatacag atgaatcaaa ttgttggtct tggcatttca acacagagag 361 caacttttat tacgtggaac aagaaaacag gaaatcattt tcacaacttt ataagttggc 421 aagacttaag agctgttgaa cttgtaaaat cttggaataa ttctcttctt atgaagatat 481 ttcacagttc ttgccgagtg cttcactttt tcactagaag taaacgactt tttacagcca 541 gtttgttcac tttcacaacc cagcagactt ctttgagatt ggtctggatt ttacagaact 601 tgactgaggt gcaaaaggca gttgaagaag aaaattgctg ctttgggact attgatacct 661 ggttgttata taagctcaca aaaggttctg tatatgccac agatttttca aatgctagta 721 caactggact ttttgaccca tataagatgt gttggagtgg gatgattacc tctctaattt 781 cgataccact ttctctccta cctcctgtga gggacacaag ccacaatttt ggatcagtgg 841 atgaagagat atttggtgtg cctataccaa tagttgcctt gttaaaggtc cctggatatg 901 accagaatat ctgctatatt tttgggaagg ggaccattga gccagtattc tatcatggaa 961 gtactttata cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1021 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1081 tcaacctctg gatta