Construct: ORF ccsbBroad304_10359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001621.1_s304c1, BRDN0000410788
- Derived from:
- ccsbBroadEn_10359
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECEL1P2 (347694)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10359
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 347694 | ECEL1P2 | endothelin converting enzym... | NR_028501.1 | 9.8% | 1_125del;255_1309del | |
| 2 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_001290787.2 | 4.9% | 4.6% | (many diffs) |
| 3 | human | 9427 | ECEL1 | endothelin converting enzym... | NM_004826.4 | 4.9% | 4.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 195
- ORF length:
- 129
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg cgagatcgaa cgactgaacc cgctgctcat gctggaggtc atcgaggact 121 gcgggggctg ggacctgcgc ggcgcggcgg agcgccgggg ggttgctgcg cgatgggacc 181 tcaactggct gctgtaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 241 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 301 cgacaatcaa cctctggatt a