Construct: ORF ccsbBroad304_10420

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003651.1_s304c1, BRDN0000410960
Derived from:
ccsbBroadEn_10420
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WASH8P (100288778)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10420
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100287171 WASHC1 WASH complex subunit 1 XM_024447370.1 97.9% 95.4% (many diffs)
2 human 100287171 WASHC1 WASH complex subunit 1 XM_017014171.1 67.8% 61.4% (many diffs)
3 human 100287171 WASHC1 WASH complex subunit 1 XM_017014172.1 67.8% 61.4% (many diffs)
4 human 100287171 WASHC1 WASH complex subunit 1 XM_011517666.2 58.7% 57.2% (many diffs)
5 human 100287171 WASHC1 WASH complex subunit 1 XM_011517665.2 58.6% 57.1% (many diffs)
6 human 100287171 WASHC1 WASH complex subunit 1 XM_011517664.3 57.3% 55.8% (many diffs)
7 human 100287171 WASHC1 WASH complex subunit 1 XM_017014170.1 57.3% 55.8% (many diffs)
8 human 100287171 WASHC1 WASH complex subunit 1 XM_011517663.3 57.2% 55.7% (many diffs)
9 human 100287171 WASHC1 WASH complex subunit 1 XM_017014169.1 57.2% 55.7% (many diffs)
10 human 100287171 WASHC1 WASH complex subunit 1 XM_011517662.3 55.7% 54.3% (many diffs)
11 human 100287171 WASHC1 WASH complex subunit 1 NM_182905.5 55.6% 54.1% (many diffs)
12 human 100287171 WASHC1 WASH complex subunit 1 XM_024447369.1 55.6% 54.1% (many diffs)
13 human 100287171 WASHC1 WASH complex subunit 1 XM_011517660.2 54.6% 53.2% (many diffs)
14 human 100287171 WASHC1 WASH complex subunit 1 XM_011517659.2 54.5% 53.1% (many diffs)
15 human 100287171 WASHC1 WASH complex subunit 1 XM_011517658.2 54% 52.6% (many diffs)
16 human 102723897 WASH9P WAS protein family homolog ... XR_001737556.1 44.1% (many diffs)
17 human 100287171 WASHC1 WASH complex subunit 1 XR_002956737.1 43.6% (many diffs)
18 human 653635 WASH7P WASP family homolog 7, pseu... NR_024540.1 43.3% (many diffs)
19 human 100288778 WASH8P WAS protein family homolog ... NR_130745.1 41.7% (many diffs)
20 human 100287171 WASHC1 WASH complex subunit 1 XR_002956741.1 41.1% (many diffs)
21 human 375260 WASH2P WASP family homolog 2, pseu... NR_024077.2 39.3% (many diffs)
22 human 100287171 WASHC1 WASH complex subunit 1 XR_929142.2 36.2% (many diffs)
23 human 374666 WASH3P WASP family homolog 3, pseu... NR_003659.2 30.1% (many diffs)
24 human 100287171 WASHC1 WASH complex subunit 1 XR_002956738.1 20.8% (many diffs)
25 human 100287171 WASHC1 WASH complex subunit 1 XM_017014173.1 17.9% 7.6% (many diffs)
26 human 100287171 WASHC1 WASH complex subunit 1 XR_002956739.1 10.2% (many diffs)
27 human 100287171 WASHC1 WASH complex subunit 1 XR_002956740.1 10.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
858
ORF length:
792
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gggggcagag acagaggaga agctgtttga tgcccccttg tccatcagca
121agagagagca gctggaacag caggtcccag agaactactt ctatgtgcca gacctgggcc
181aggtgcctga gattgatgtt ccatcctacc tgcctgacct gcccagcatt gccaacgacc
241tcatgtacat tgccgacctg ggccccggca ttgccccctc tgcccctggc accattccag
301aactgcccac cttccacact gaggtagccg agcctctcaa ggcagaccta caagatgggg
361tactaacacc acccccaccg cccccaccac cacccccagc tcctgaggtg ctggccagtg
421cacccccact cccaccctca accgcggccc ctgtaggcca aggcgccagg caggacgaca
481gcagcagcag cacgtctcct tcagtccagg gagctcccag ggaagtggtc gacccctcca
541gtggctgggc cactctgcta gagtccatcc gccaagctgg gggcatcggc aaggccaagc
601tgcgcagcat gaaggagcga aagctggaga agaagaagca gaaggagcag gagcaagtga
661gagccacgag ccaaggtggg cacttgatgt cggatctctt caacaagctg gtcatgaggc
721gcaagggcat ctctgggaaa ggacctgggg ctggtgaggg gcccggagga gcctttgccc
781gcgtgtcaga ctccatccct cctctgccgc caccgcagca gccacaggta gatgaggaca
841aggacgactg ggaatcctac ccaactttct tgtacaaagt ggttggtaag cctatcccta
901accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
961agtcgacaat caacctctgg atta

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