Transcript: Human XM_017014173.1

PREDICTED: Homo sapiens WASH complex subunit 1 (WASHC1), transcript variant X21, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WASHC1 (100287171)
Length:
1154
CDS:
219..989

Additional Resources:

NCBI RefSeq record:
XM_017014173.1
NBCI Gene record:
WASHC1 (100287171)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017014173.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000245681 AGGAGAAGCTGAAGGACTTTC pLKO_005 667 CDS 100% 10.800 5.400 Y WASH5P n/a
2 TRCN0000168461 GTCCCAGAGAACTACTTCTAT pLKO.1 806 CDS 100% 5.625 2.813 Y WASH3P n/a
3 TRCN0000173054 CCAGGCCAAGATTGAGAAGAT pLKO.1 476 CDS 100% 4.950 2.475 Y WASH3P n/a
4 TRCN0000127606 CCCAGAGAACTACTTCTATGT pLKO.1 808 CDS 100% 4.950 2.475 Y WASH2P n/a
5 TRCN0000129470 GAAGATCAAGGGCAGCAAGAA pLKO.1 491 CDS 100% 4.950 2.475 Y WASH2P n/a
6 TRCN0000129376 GCAGGAATATGGCTCCATCTT pLKO.1 560 CDS 100% 4.950 2.475 Y WASH2P n/a
7 TRCN0000245682 AGACATCTTCAGCAGGATCTC pLKO_005 395 CDS 100% 4.050 2.025 Y WASH5P n/a
8 TRCN0000127771 GAGAACTACTTCTATGTGCCA pLKO.1 812 CDS 100% 0.660 0.330 Y WASH2P n/a
9 TRCN0000172980 GCCAACGACCTCATGTACATT pLKO.1 893 CDS 100% 5.625 2.813 Y WASH5P n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017014173.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13628 pDONR223 100% 49.5% 41.2% None (many diffs) n/a
2 ccsbBroad304_13628 pLX_304 0% 49.5% 41.2% V5 (many diffs) n/a
3 TRCN0000468550 ACATGGAGTCAGTTCTTTTTGGCA pLX_317 30.2% 49.5% 41.2% V5 (many diffs) n/a
4 ccsbBroadEn_10420 pDONR223 100% 17.9% 7.6% None (many diffs) n/a
5 ccsbBroad304_10420 pLX_304 0% 17.9% 7.6% V5 (many diffs) n/a
6 TRCN0000475386 CCTTCTGTACATCGGAATTTACAA pLX_317 33% 17.9% 7.6% V5 (many diffs) n/a
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