Construct: ORF ccsbBroad304_10424
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018306.1_s304c1, BRDN0000403514
- Derived from:
- ccsbBroadEn_10424
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPA2 (1358)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10424
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1358 | CPA2 | carboxypeptidase A2 | NM_001869.3 | 99.2% | 99.2% | (many diffs) |
| 2 | mouse | 232680 | Cpa2 | carboxypeptidase A2, pancre... | NM_001024698.3 | 85.4% | 86% | (many diffs) |
| 3 | mouse | 232680 | Cpa2 | carboxypeptidase A2, pancre... | XM_011241057.1 | 65.5% | 67.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1317
- ORF length:
- 1251
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gttgatcctg ttttttggtg ccctttttgg gcatatctac tgtctagaaa 121 catttgtggg agaccaagtt cttgagattg taccaagcaa tgaagaacaa attaaaaatc 181 tgctacaatt ggaggctcaa gaacatctcc agcttgattt ttggaaatca cccaccaccc 241 caggggagac agcccacgtc cgagttccct tcgtcaacgt ccaggcagtc aaagtgttct 301 tggggtccca gggaattgcc tattccatca tgattgaaga cgtgcaggtc ctgttggaca 361 aagagaatga agaaatgctt tttaatagga gaagagaacg gagtggtaac ttcaattttg 421 gggcctacca taccctggaa gagatttccc aagaaatgga taacctcgtg gctgagcacc 481 ccggtctagt gagcaaagtg aatattggct cttcttttga gaaccggcct atgaacgtgc 541 tcaagttcag caccggagga gacaagccag ctatctggct ggatgctggg atccatgctc 601 gagagtgggt tacacaagct acggcacttt ggacagcaaa taagattgtt tctgattatg 661 gaaaggaccc atccatcact tccattctgg acgccctgga tatcttcctc ctgccagtca 721 caaaccctga tggatacgtg ttctctcaaa ccaaaaatcg tatgtggcgg aagacccggt 781 ccaaggtatc tggaagcctc tgtgttggtg tggatcctaa ccggaactgg gatgcaggtt 841 ttggaggacc tggagccagc agcaaccctt gctctgattc ataccacgga cccagtgcca 901 actctgaagt tgaagtgaaa tccatagtgg acttcatcaa gagtcatgga aaagtcaagg 961 ccttcattac cctccacagc tattcccagc tgctgatgtt cccctatggg tacaaatgta 1021 ccaagttaga tgactttgat gagctgagtg aagtggccca aaaggctgcc caatctctga 1081 gaagcctgca tggcaccaag tacaaagtgg gaccaatctg ctctgtcatc taccaagcca 1141 gtggaggaag cattgactgg tcctatgatt atggcatcaa gtactcattt gcctttgaac 1201 tgagagacac agggcgctac ggcttcctct tgccagcccg tcagatcctg cccacagccg 1261 aggagacctg gcttggcttg aaggcaatca tggagcatgt gcgagaccac ccctattacc 1321 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1381 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1441 tta