Construct: ORF ccsbBroad304_10443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003328.1_s304c1, BRDN0000408081
- Derived from:
- ccsbBroadEn_10443
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPA1 (6117)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10443
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6117 | RPA1 | replication protein A1 | NM_002945.5 | 99.8% | 99.8% | 1056C>T;1846A>G |
| 2 | human | 6117 | RPA1 | replication protein A1 | NM_001355120.2 | 97.7% | 97.7% | 0_1ins39;1017C>T;1807A>G |
| 3 | human | 6117 | RPA1 | replication protein A1 | XM_024450863.1 | 92.4% | 89.4% | (many diffs) |
| 4 | human | 6117 | RPA1 | replication protein A1 | NM_001355121.2 | 90.3% | 90.2% | 1056C>T;1374_1375ins177;1669A>G |
| 5 | mouse | 68275 | Rpa1 | replication protein A1 | NM_026653.2 | 83.6% | 84.3% | (many diffs) |
| 6 | mouse | 68275 | Rpa1 | replication protein A1 | NM_001164223.1 | 80.9% | 81.6% | (many diffs) |
| 7 | mouse | 68275 | Rpa1 | replication protein A1 | XM_006534051.1 | 79.9% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1914
- ORF length:
- 1848
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaataag 61 tcggcatggt cggccaactg agcgaggggg ccattgcggc catcatgcag aagggggata 121 caaacataaa gcccatcctc caagtcatca acatccgtcc cattactacg gggaatagtc 181 cgccgcgtta tcgactgctc atgagtgatg gattgaacac tctatcctct ttcatgttgg 241 cgacacagtt gaaccctctc gtggaggaag aacaattgtc cagcaactgt gtatgccaga 301 ttcacagatt tattgtgaac actctgaaag acggaaggag agtagttatc ttgatggaat 361 tagaagtttt gaagtcagct gaagcagttg gagtgaagat tggcaatcca gtgccctata 421 atgaaggact cgggcagccg caagtagctc ctccagcgcc agcagccagc ccagcagcaa 481 gcagcaggcc ccagccgcag aatggaagct cgggaatggg ttctactgtt tctaaggctt 541 atggtgcttc aaagacattt ggaaaagctg caggtcccag cctgtcacac acttctgggg 601 gaacacagtc caaagtggtg cccattgcca gcctcactcc ttaccagtcc aagtggacca 661 tttgtgctcg tgttaccaac aaaagtcaga tccgtacctg gagcaactcc cgaggggaag 721 ggaagctttt ctccctagaa ctggttgacg aaagtggtga aatccgagct acagctttca 781 atgagcaagt ggacaagttc tttcctctta ttgaagtgaa caaggtgtat tatttctcga 841 aaggcaccct gaagattgct aacaagcagt tcacagctgt taaaaatgac tacgagatga 901 ccttcaataa cgagacttcc gtcatgccct gtgaggacga ccatcattta cctacggttc 961 agtttgattt cacggggatt gatgacctcg agaacaagtc gaaagactca cttgtagaca 1021 tcatcgggat ctgcaagagc tatgaagacg ccactaaaat cacagtgagg tctaacaaca 1081 gagaagttgc caagaggaat atctacttga tggacacatc tgggaaggtg gtgactgcta 1141 cactgtgggg ggaagatgct gataaatttg atggttctag acagcccgtg ttggctatca 1201 aaggagcccg agtctctgat ttcggtggac ggagcctctc cgtgctgtct tcaagcacta 1261 tcattgcgaa tcctgacatc ccagaggcct ataagcttcg tggatggttt gacgcagaag 1321 gacaagcctt agatggtgtt tccatctctg atctaaagag cggcggagtc ggagggagta 1381 acaccaactg gaaaaccttg tatgaggtca aatccgagaa cctgggccaa ggcgacaagc 1441 cggactactt tagttctgtg gccacagtgg tgtatcttcg caaagagaac tgcatgtacc 1501 aagcctgccc gactcaggac tgcaataaga aagtgattga tcaacagaat ggattgtacc 1561 gctgtgagaa gtgcgacacc gaatttccca atttcaagta ccgcatgatc ctgtcagtaa 1621 atattgcaga ttttcaagag aatcagtggg tgacttgttt ccaggagtct gctgaagcta 1681 tccttggaca aaatgctgct tatcttgggg aattaaaaga caagaatgaa caggcatttg 1741 aagaagtttt ccagaatgcc aacttccgat ctttcatatt cagagtcagg gtcaaagtgg 1801 agacctacaa cgacgagtct cgaattaagg ccactgtgat ggacgtgaag cccgtggact 1861 acagagagta tggccgaagg ctggtcatga gcatcaggag aagtgcattg gtgtgcccaa 1921 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1981 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta