Construct: ORF ccsbBroad304_10464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007382.1_s304c1, BRDN0000461644
- Derived from:
- ccsbBroadEn_10464
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSKS (60385)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10464
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60385 | TSKS | testis specific serine kina... | NM_021733.2 | 99.4% | 99.4% | 1768_1776delCCTGAACAA |
| 2 | human | 60385 | TSKS | testis specific serine kina... | XM_011527201.1 | 95.4% | 90% | 1497_1539del;1666_1697del;1843_1851delCCTGAACAA |
| 3 | human | 60385 | TSKS | testis specific serine kina... | XM_011527202.1 | 93.7% | 82.3% | 1497_1539del;1740_1741ins70 |
| 4 | human | 60385 | TSKS | testis specific serine kina... | XM_011527203.2 | 91.8% | 91.8% | 1623_1624ins144 |
| 5 | mouse | 22116 | Tsks | testis-specific serine kina... | XM_006540794.3 | 81.8% | 82.6% | (many diffs) |
| 6 | mouse | 22116 | Tsks | testis-specific serine kina... | NM_011651.2 | 80.6% | 81.3% | (many diffs) |
| 7 | mouse | 22116 | Tsks | testis-specific serine kina... | XM_006540792.3 | 78.5% | 73.7% | (many diffs) |
| 8 | mouse | 22116 | Tsks | testis-specific serine kina... | XM_006540791.3 | 77.4% | 72.6% | (many diffs) |
| 9 | mouse | 22116 | Tsks | testis-specific serine kina... | XM_006540795.3 | 76.2% | 69.1% | (many diffs) |
| 10 | mouse | 22116 | Tsks | testis-specific serine kina... | NM_001077591.1 | 75.5% | 77% | (many diffs) |
| 11 | mouse | 22116 | Tsks | testis-specific serine kina... | XM_006540796.3 | 74.6% | 69.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1833
- ORF length:
- 1767
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gagcgtggtg gtgaagacga tctggcagtc caaagagatc catgaggccg 121 gggacacccc cacgggggtg gagagctgct cccagctagt cccagaggct ccccggaggg 181 tgaccagccg ggccaagggg atcccgaaga aaaagaaggc cgtgtcgttc cacggggtgg 241 agccccagat gtcccatcag cccatgcact ggtgcctgaa cctcaaacgg tcctcggcct 301 gcaccaacgt gtcactgctc aacctggccg ccatggagcc cactgactcc acggggacag 361 actccacagt ggaagacctc agcggccaac tcacactggc tgggccccct gcctccccta 421 ccctaccctg ggatccggat gacgcagaca tcacggaaat cctgagtggg gtcaacagtg 481 gattggtccg cgccaaagac tccatcacca gcttgaagga aaagaccaac cgggttaacc 541 agcacgtgca gtctctgcag agcgagtgtt ctgtgctgag cgagaatctg gagagaaggc 601 ggcaagaggc agaagagttg gaggggtact gcattcaact caaggagaac tgctggaagg 661 tgacccggtc tgtggaagat gctgaaatca aaaccaacgt cttgaagcag aattctgccc 721 tgctggagga gaagctgcgc tacctccagc agcagctgca ggatgagacg ccgcgacggc 781 aggaggccga gctgcaggag ccggaggaga agcaggagcc ggaggagaag caggagccgg 841 aggagaagca gaagccggag gctggcctct cctggaacag cctgggcccc gccgccacgt 901 cccagggctg ccccggcccg ccagggagtc ccgacaaacc ctcgcggcca cacggcctgg 961 tccccgcagg ctggggaatg gggcctcggg ctggcgaggg cccctacgtg agcgagcagg 1021 aattgcagaa gctgttcacc ggcatcgaag agctgaggag agaggtgtcc tcactgaccg 1081 cccggtggca tcaggaggag ggggcggtgc aggaagccct gcggctgctc gggggcctgg 1141 gcggcagggt cgacggcttc ctaggccagt gggagcgggc acagcgcgaa caggcacaga 1201 cggcgcggga cttgcaggag ctgcgaggtc gggcggatga gctgtgcacc atggtggagc 1261 ggtcagcagt gtctgtggct tcactgagga gcgaactgga ggggctgggc ccactgaaac 1321 ccattctgga ggagttcggg cggcaatttc agaactctcg aagagggcct gacctctcca 1381 tgaacctgga tcggtcccac caaggcaact gtgcccgctg tgccagccag gggtcgcagt 1441 tgtctacgga gtccctgcag cagctgctgg accgagcact gacctcacta gtggacgagg 1501 tgaagcagag gggcctgact cctgcctgtc ccagctgtca gaggctacac aagaagattc 1561 tggagctgga gcgccaggcc ttagccaaac acgtcagggc agaggccctg agctccaccc 1621 ttcggctggc ccaagacgag gccctgcggg ccaagaacct actgctgaca gacaagatga 1681 agccagagga gaagatggcc actctggacc atctacactt gaagatgtgc tccctccacg 1741 atcatctcag caacctgcca cttgaggggt ccacgggaac aatgggggga ggcagcagtg 1801 caggaacccc cccaaaacag gggggctcag cctgcccaac tttcttgtac aaagtggttg 1861 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1921 accggtggcg cgttaagtcg acaatcaacc tctggatta