Construct: ORF ccsbBroad304_10619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008717.1_s304c1, BRDN0000408121
- Derived from:
- ccsbBroadEn_10619
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRIM73 (375593)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10619
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744741.2 | 13.1% | 1_1363del;1601_1806del | |
| 2 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_002956439.1 | 7.3% | 1_1594del;1832_3215del | |
| 3 | human | 378108 | TRIM74 | tripartite motif containing 74 | XR_001744731.2 | 7% | 1_2903del;3141_3346del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 303
- ORF length:
- 237
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc cggcagaccc ctaccgacgg cggcattcca ttctggggcc acgaggaggc 121 actcatcgtt tcgttccaac cgccgccaac ggccctggcc ctcgcgagct ctggaactac 181 agaggtcgca cggtgagttg ccaggtgtgg cccgtaatcg gagcgcacaa aacatgatgg 241 gacacgtaac gggaccacac agggcacatt gggcacttgc aggggcgcga ggtggcggca 301 cgtacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 361 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 421 tctggatta