Construct: ORF ccsbBroad304_10695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004333.1_s304c1, BRDN0000402523
- Derived from:
- ccsbBroadEn_10695
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCHE (590)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10695
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 590 | BCHE | butyrylcholinesterase | NR_137635.2 | 21.8% | 1_207del;400_880del | |
| 2 | human | 590 | BCHE | butyrylcholinesterase | NM_000055.4 | 10.6% | 10.6% | 1_1614del |
| 3 | human | 590 | BCHE | butyrylcholinesterase | NR_137636.2 | 7.7% | 1_1732del;1803_1881del;2004_2484del | |
| 4 | mouse | 12038 | Bche | butyrylcholinesterase | NM_009738.3 | 9.1% | 8.4% | (many diffs) |
| 5 | mouse | 12038 | Bche | butyrylcholinesterase | XM_011240000.2 | 8.9% | 8.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 258
- ORF length:
- 192
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac gaaactacgt gctcaacaat gtcgattctg gacatcattt tttccaaaag 121 tcttggaaat gacaggaaat attgatgaag cagaatggga gtggaaagca ggattccatc 181 gctggaacaa ttacatgatg gactggaaaa atcaatttaa cgattacact agcaagaaag 241 aaagttgtgt gggtctctac ccaactttct tgtacaaagt ggttggtaag cctatcccta 301 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 361 agtcgacaat caacctctgg atta