Construct: ORF ccsbBroad304_10716

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005985.1_s304c1, BRDN0000408776
Derived from:
ccsbBroadEn_10716
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CD22 (933)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10716
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 933 CD22 CD22 molecule NM_001278417.2 100% 100%
2 human 933 CD22 CD22 molecule NM_001771.4 79.6% 79.3% (many diffs)
3 human 933 CD22 CD22 molecule NM_001185099.2 69.2% 68.9% (many diffs)
4 human 933 CD22 CD22 molecule NM_001185100.2 68.2% 66.3% (many diffs)
5 human 933 CD22 CD22 molecule NM_001185101.1 58.7% 58.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2094
ORF length:
2025
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtctctgtgg ctcctagagg gggttccaat gaggcaggct gctgtcacct
121cgacctcctt gaccatcaag tctgtcttca cccggagcga gctcaagttc tccccacagt
181ggagtcacca tgggaagatt gtgacctgcc agcttcagga tgcagatggg aagttcctct
241ccaatgacac ggtgcagctg aacgtgaagc acaccccgaa gttggagatc aaggtcactc
301ccagtgatgc catagtgagg gagggggact ctgtgaccat gacctgcgag gtcagcagca
361gcaacccgga gtacacgacg gtatcctggc tcaaggatgg gacctcgctg aagaagcaga
421atacattcac gctaaacctg cgcgaagtga ccaaggacca gagtgggaag tactgctgtc
481aggtctccaa tgacgtgggc ccgggaaggt cggaagaagt gttcctgcaa gtgcagtatg
541ccccggaacc ttccacggtt cagatcctcc actcaccggc tgtggaggga agtcaagtcg
601agtttctttg catgtcactg gccaatcctc ttccaacaaa ttacacgtgg taccacaatg
661ggaaagaaat gcagggaagg acagaggaga aagtccacat cccaaagatc ctcccctggc
721acgctgggac ttattcctgt gtggcagaaa acattcttgg tactggacag aggggcccgg
781gagctgagct ggatgtccag tatcctccca agaaggtgac cacagtgatt caaaacccca
841tgccgattcg agaaggagac acagtgaccc tttcctgtaa ctacaattcc agtaacccca
901gtgttacccg gtatgaatgg aaaccccatg gcgcctggga ggagccatcg cttggggtgc
961tgaagatcca aaacgttggc tgggacaaca caaccatcgc ctgcgcagct tgtaatagtt
1021ggtgctcgtg ggcctcccct gtcgccctga atgtccagta tgccccccga gacgtgaggg
1081tccggaaaat caagcccctt tccgagattc actctggaaa ctcggtcagc ctccaatgtg
1141acttctcaag cagccacccc aaagaagtcc agttcttctg ggagaaaaat ggcaggcttc
1201tggggaaaga aagccagctg aattttgact ccatctcccc agaagatgct gggagttaca
1261gctgctgggt gaacaactcc ataggacaga cagcgtccaa ggcctggaca cttgaagtgc
1321tgtatgcacc caggaggctg cgtgtgtcca tgagcccggg ggaccaagtg atggagggga
1381agagtgcaac cctgacctgt gagagcgacg ccaaccctcc cgtctcccac tacacctggt
1441ttgactggaa taaccaaagc ctcccctacc acagccagaa gctgagattg gagccggtga
1501aggtccagca ctcgggtgcc tactggtgcc aggggaccaa cagtgtgggc aagggccgtt
1561cgcctctcag caccctcacc gtctactata gcccggagac catcggcagg cgagtggctg
1621tgggactcgg gtcctgcctc gccatcctca tcctggcaat ctgtgggctc aagctccagc
1681gacgttggaa gaggacacag agccagcagg ggcttcagga gaattccagc ggccagagct
1741tctttgtgag gaataaaaag gttagaaggg cccccctctc tgaaggcccc cactccctgg
1801gatgctacaa tccaatgatg gaagatggca ttagctacac caccctgcgc tttcccgaga
1861tgaacatacc acgaactgga gatgcagagt cctcagagat gcagagacct cccccggact
1921gcgatgacac ggtcacttat tcagcattgc acaagcgcca agtgggcgac tatgagaacg
1981tcattccaga ttttccagaa gatgagggga ttcattactc agagctgatc cagtttgggg
2041tcggggagcg gcctcaggca caagaaaatg tggactatgt gatcctcaaa catttgccaa
2101ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
2161cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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