Construct: ORF ccsbBroad304_10752
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007314.1_s304c1, BRDN0000403864
- Derived from:
- ccsbBroadEn_10752
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRY2 (1408)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10752
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1408 | CRY2 | cryptochrome circadian regu... | NM_021117.5 | 100% | 100% | |
| 2 | human | 1408 | CRY2 | cryptochrome circadian regu... | NM_001127457.2 | 89.3% | 88% | (many diffs) |
| 3 | mouse | 12953 | Cry2 | cryptochrome 2 (photolyase-... | NM_009963.4 | 90.2% | 94.2% | (many diffs) |
| 4 | mouse | 12953 | Cry2 | cryptochrome 2 (photolyase-... | XM_017315281.1 | 90.2% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1845
- ORF length:
- 1779
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgactgtg gcgacggcgg cagctgtggc cccggcgcca gcgcccggca 121 cggacagcgc ctcttcggtg cactggttcc gcaaagggct gcgactccac gacaacccgg 181 cgttgctggc ggccgtgcgc ggggcgcgct gcgtgcgctg cgtttacatt ctcgacccgt 241 ggttcgcggc ctcctcctca gtcgggatca accgatggag gttcctactt cagtctctgg 301 aagatttgga cacaagttta aggaaactga actcccgcct gtttgtagtc cggggacagc 361 cagccgacgt gttcccaagg ctgttcaagg aatggggagt gacccgcttg acctttgaat 421 atgactctga accctttggg aaagaacggg atgcagccat catgaagatg gccaaggagg 481 ctggtgtgga agtagtgacg gagaattctc ataccctcta tgacctggac aggatcattg 541 agctgaatgg gcagaagcca ccccttacat acaagcgctt tcaggccatc atcagccgca 601 tggagctgcc caagaagcca gtgggcttgg tgaccagcca gcagatggag agctgcaggg 661 ccgagatcca ggagaaccac gacgagacct acggcgtgcc ctccctggag gagctggggt 721 tccccactga aggacttggt ccagctgtct ggcagggagg agagacagaa gctctggccc 781 gcctggataa gcacttggaa cggaaggcct gggttgccaa ctatgagaga ccccgaatga 841 acgccaactc cctcctggcc agccccacag gcctcagccc ctacctgcgc tttggttgtc 901 tctcctgccg cctcttctac taccgcctgt gggacctgta taaaaaggtg aagcggaaca 961 gcacacctcc cctctcccta tttgggcaac tcctatggcg agagttcttc tacacggcag 1021 ctaccaacaa ccccaggttt gaccgcatgg aggggaaccc catctgcatc cagatcccct 1081 gggaccgcaa tcctgaggcc ctggccaagt gggctgaggg caagacaggc ttcccttgga 1141 ttgatgccat catgacccaa ctgaggcagg agggctggat ccaccacctg gcccggcatg 1201 ccgtggcctg cttcctgacc cgcggggacc tctgggtcag ctgggagagc ggggtccggg 1261 tatttgatga gctgctcctg gatgcagatt tcagcgtgaa cgcaggcagc tggatgtggc 1321 tgtcctgcag tgctttcttc cagcagttct tccactgcta ctgccctgtg ggctttggcc 1381 gtcgcacgga ccccagtggg gactacatca ggcgatacct gcccaaattg aaagcgttcc 1441 cctctcgata catctatgag ccctggaatg ccccagagtc aattcagaag gcagccaagt 1501 gcatcattgg tgtggactac ccacggccca tcgtcaacca tgccgagacc agccggctta 1561 acattgaacg aatgaagcag atttaccagc agctttcgcg ctaccgggga ctctgtctac 1621 tggcatctgt cccttcctgt gtggaagacc tcagtcaccc tgtggcagag cccagctcga 1681 gccaggctgg cagcatgagc agtgcaggcc caagaccact acccagtggc ccagcatccc 1741 ccaaacgcaa gctggaagca gccgaggaac cacctggtga agaactcagc aaacgggccc 1801 gggtggcaga gttgccaacc ccagagctgc cgagcaagga tgcctgccca actttcttgt 1861 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1921 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a