Transcript: Mouse XM_017315281.1

PREDICTED: Mus musculus cryptochrome 2 (photolyase-like) (Cry2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Cry2 (12953)
Length:
1984
CDS:
192..1970

Additional Resources:

NCBI RefSeq record:
XM_017315281.1
NBCI Gene record:
Cry2 (12953)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017315281.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000194121 GCTCAACATTGAACGAATGAA pLKO.1 1679 CDS 100% 5.625 7.875 N Cry2 n/a
2 TRCN0000173676 CGGATGAATGCCAATTCCTTA pLKO.1 957 CDS 100% 4.950 6.930 N Cry2 n/a
3 TRCN0000176196 GAATATGACTCTGAACCCTTT pLKO.1 540 CDS 100% 4.050 5.670 N Cry2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017315281.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10752 pDONR223 100% 90.2% 94.2% None (many diffs) n/a
2 ccsbBroad304_10752 pLX_304 0% 90.2% 94.2% V5 (many diffs) n/a
3 TRCN0000487818 ATCAGAGCCACGGTGCAACCACGT pLX_317 11.3% 90.2% 94.2% V5 (many diffs) n/a
4 TRCN0000489843 AGAATTGCATCTTTGGTCTGTATC pLX_317 26.2% 90.2% 94.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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