Transcript: Human NM_021117.5

Homo sapiens cryptochrome circadian regulator 2 (CRY2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
CRY2 (1408)
Length:
4131
CDS:
17..1798

Additional Resources:

NCBI RefSeq record:
NM_021117.5
NBCI Gene record:
CRY2 (1408)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021117.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000307285 CCCTTACATACAAGCGCTTTC pLKO_005 513 CDS 100% 6.000 8.400 N CRY2 n/a
2 TRCN0000078299 CCGGCTTAACATTGAACGAAT pLKO.1 1504 CDS 100% 4.950 6.930 N CRY2 n/a
3 TRCN0000078302 GTGGAAGTAGTGACGGAGAAT pLKO.1 437 CDS 100% 4.950 6.930 N CRY2 n/a
4 TRCN0000286835 GTGGAAGTAGTGACGGAGAAT pLKO_005 437 CDS 100% 4.950 6.930 N CRY2 n/a
5 TRCN0000078301 CCTGCCCAAATTGAAAGCGTT pLKO.1 1369 CDS 100% 2.640 3.696 N CRY2 n/a
6 TRCN0000078298 GCCCAGATTTAGTATTCACTA pLKO.1 3675 3UTR 100% 4.950 3.960 N CRY2 n/a
7 TRCN0000294220 CGCCTGTGGGACCTGTATAAA pLKO_005 875 CDS 100% 15.000 10.500 N CRY2 n/a
8 TRCN0000294219 ATCTGTTGACACTCATGATTC pLKO_005 1969 3UTR 100% 10.800 7.560 N CRY2 n/a
9 TRCN0000176196 GAATATGACTCTGAACCCTTT pLKO.1 368 CDS 100% 4.050 2.835 N Cry2 n/a
10 TRCN0000078300 CACAAGTTTAAGGAAACTGAA pLKO.1 262 CDS 100% 0.495 0.347 N CRY2 n/a
11 TRCN0000306873 AGGCAGCCAAGTGCATCATTG pLKO_005 1440 CDS 100% 10.800 6.480 N CRY2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021117.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10752 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_10752 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000487818 ATCAGAGCCACGGTGCAACCACGT pLX_317 11.3% 100% 100% V5 n/a
4 TRCN0000489843 AGAATTGCATCTTTGGTCTGTATC pLX_317 26.2% 100% 100% V5 (not translated due to prior stop codon) n/a
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