Construct: ORF ccsbBroad304_10778
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012359.1_s304c1, BRDN0000405283
- Derived from:
- ccsbBroadEn_10778
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIAPH2 (1730)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10778
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1730 | DIAPH2 | diaphanous related formin 2 | NM_007309.3 | 99.3% | 99.3% | 447_448ins21 |
| 2 | human | 1730 | DIAPH2 | diaphanous related formin 2 | NM_006729.5 | 98% | 97.3% | (many diffs) |
| 3 | mouse | 54004 | Diaph2 | diaphanous related formin 2 | XM_006528581.3 | 83.7% | 82.7% | (many diffs) |
| 4 | mouse | 54004 | Diaph2 | diaphanous related formin 2 | NM_172493.2 | 83.1% | 82.2% | (many diffs) |
| 5 | mouse | 54004 | Diaph2 | diaphanous related formin 2 | XM_006528582.3 | 82.7% | 81.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3378
- ORF length:
- 3309
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaagat ggagcagccc ggggcggcgg cgtcgggagc gggaggcggc agcgaggaac 121 ccggtggggg ccggagcaac aagcggagcg cggggaaccg ggccgccaat gaagaggaaa 181 cgaaaaacaa acccaaattg aacattcaaa taaaaacttt ggcagatgat gtgcgtgacc 241 gaattacaag ttttagaaaa tctactgtca aaaaagaaaa acctcttatt caacatccta 301 ttgattctca agtcgcgatg agtgagtttc ctgcagctca gccattatat gatgaacgat 361 ctttgaattt gtcagaaaag gaagtattgg atctctttga aaaaatgatg gaggacatga 421 accttaacga agagaaaaaa gctcctttac gaaacaaaga ctttaccacc aaacgtgaga 481 tggttgtcca gtatatttct gccactgcca aatctatagt tggaagtaaa gttacgggtg 541 ggctgaaaaa cagcaaacat gaatgcaccc tgtcttcaca agaatatgtt catgaattac 601 gatcgggtat atcagatgag aaacttctta attgcctaga atccctcagg gtttctttaa 661 ccagcaatcc ggtcagctgg gttaacaact ttggccatga aggtcttgga ctcttattgg 721 atgagctgga aaagcttctg gacaaaaaac agcaagaaaa tattgacaag aagaatcagt 781 ataaacttat tcaatgcctc aaagcattta tgaataataa gtttggatta caaaggattc 841 taggagatga aagaagtctt ttactattgg caagagcaat tgaccccaaa caacccaaca 901 tgatgactga aatagtaaaa atactttctg ctatttgcat tgttggagaa gagaacattc 961 tagataaact tttaggggct ataacaacag cagcagaaag aaataacagg gaacgatttt 1021 caccaattgt ggaaggttta gaaaatcagg aagccttgca attacaggtg gcctgcatgc 1081 agtttataaa tgcccttgtc acttctcctt atgagcttga ttttcgaata catttaagga 1141 atgaattcct ccgttcagga ctaaaaacaa tgttaccaga tctaaaagaa aaagagaatg 1201 atgagcttga tattcagttg aaagtatttg atgaaaacaa agaagatgac ctaactgaat 1261 tatcacaccg tctcaatgac attcgagcag aaatggatga tatgaatgaa gtctaccatc 1321 ttctatataa tatgctgaag gacactgctg ctgaaaatta cttcttatct attctacaac 1381 attttttgct tatcagaaat gattattata tcaggccaca atattataaa ataattgagg 1441 aatgtgtttc acagatagtg ctacactgca gtggtatgga tccagacttc aaatacaggc 1501 aaagattaga catcgattta actcatctga tagattcttg tgtgaacaag gcgaaagttg 1561 aagaaagtga acaaaaagct gcagagtttt caaagaagtt cgatgaagaa ttcacagctc 1621 gacaggaagc tcaagcagag cttcaaaaaa gagatgagaa aatcaaagaa cttgaagcag 1681 aaatccagca acttcgaacc caggcacaag tactctcaag ttcatcagga attccaggtc 1741 ctcctgcagc acctccattg ccaggtgtag ggccgcctcc accaccaccc gcgccacctc 1801 tacccggagg agctcctctt cctcctccac cacctccttt acctggaatg atggggatac 1861 caccaccacc cccaccacca cttttatttg ggggacctcc tccaccacca ccccttggag 1921 gagttcctcc tcccccagga atatcactta atctacctta tggaatgaag cagaaaaaaa 1981 tgtataaacc tgaagtgtcc atgaagagaa tcaattggtc aaagattgaa cccacagaat 2041 tatctgagaa ctgtttctgg ttaagagtca aagaagacaa gtttgagaat ccagatctct 2101 ttgccaaatt ggcattgaat tttgctactc agataaaagt tcaaaagaac gcagaagcat 2161 tagaagaaaa gaagactggg cctacaaaga agaaagtgaa agaactgaga attttggatc 2221 ccaaaacagc tcagaatctg tccatctttc tgggatcata tcgcatgcca tatgaagaca 2281 taagaaacgt tattctggag gttaatgaag acatgctgag tgaggcttta attcagaacc 2341 ttgtgaaaca tcttcctgag cagaagatac tcaacgaatt agcagagctt aagaatgaat 2401 atgatgacct ctgtgagcct gaacaatttg gagttgtgat gagctctgtg aaaatgttac 2461 agcctcgtct cagtagtatc ctgttcaagc tcacatttga agaacacata aacaacatca 2521 aaccaagcat catagcagta actcttgcct gtgaagaact gaagaaaagt gaaagcttta 2581 acagactttt agagttagtt cttcttgttg gaaactacat gaactcaggc tcaagaaatg 2641 cccagtcttt gggatttaag atcaacttcc tttgtaagat cagagatact aaatcagcgg 2701 atcaaaaaac aacccttttg cattttattg ccgacatttg tgaggaaaaa tatcgagata 2761 tcctaaaatt tcctgaagaa ctggaacacg tagaaagtgc aagcaaagtt tcagctcaaa 2821 ttctcaagag caaccttgca tcaatggaac aacaaattgt tcatctggaa cgtgacatca 2881 agaaattccc ccaagcagaa aatcaacacg ataagtttgt ggaaaagatg accagcttta 2941 caaagactgc ccgagaacag tatgaaaaac tctccaccat gcacaacaac atgatgaagc 3001 tctatgagaa tcttggagaa tacttcattt ttgactcaaa gacagtgagc atagaagagt 3061 tctttggtga tctcaacaac ttccgaactt tgtttttgga agcagtgaga gaaaacaata 3121 agagaagaga aatggaagag aagaccagga gggcaaaact tgcaaaagag aaagctgaac 3181 aagaaaagtt agaacgccag aagaaaaaga aacaactcat tgatataaac aaagagggtg 3241 atgagactgg tgtgatggat aatcttctag aagccctaca atcaggtgca gcattcagag 3301 accgtcgaaa gcggattcca aggaatccag tggtaaatca tccctgtgca acaagggcta 3361 atccaagatc agctacattg caaactttct tgtacaaagt ggttggtaag cctatcccta 3421 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 3481 agtcgacaat caacctctgg atta