Construct: ORF ccsbBroad304_10778

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012359.1_s304c1, BRDN0000405283
Derived from:
ccsbBroadEn_10778
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DIAPH2 (1730)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10778
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1730 DIAPH2 diaphanous related formin 2 NM_007309.3 99.3% 99.3% 447_448ins21
2 human 1730 DIAPH2 diaphanous related formin 2 NM_006729.5 98% 97.3% (many diffs)
3 mouse 54004 Diaph2 diaphanous related formin 2 XM_006528581.3 83.7% 82.7% (many diffs)
4 mouse 54004 Diaph2 diaphanous related formin 2 NM_172493.2 83.1% 82.2% (many diffs)
5 mouse 54004 Diaph2 diaphanous related formin 2 XM_006528582.3 82.7% 81.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3378
ORF length:
3309
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaagat ggagcagccc ggggcggcgg cgtcgggagc gggaggcggc agcgaggaac
121ccggtggggg ccggagcaac aagcggagcg cggggaaccg ggccgccaat gaagaggaaa
181cgaaaaacaa acccaaattg aacattcaaa taaaaacttt ggcagatgat gtgcgtgacc
241gaattacaag ttttagaaaa tctactgtca aaaaagaaaa acctcttatt caacatccta
301ttgattctca agtcgcgatg agtgagtttc ctgcagctca gccattatat gatgaacgat
361ctttgaattt gtcagaaaag gaagtattgg atctctttga aaaaatgatg gaggacatga
421accttaacga agagaaaaaa gctcctttac gaaacaaaga ctttaccacc aaacgtgaga
481tggttgtcca gtatatttct gccactgcca aatctatagt tggaagtaaa gttacgggtg
541ggctgaaaaa cagcaaacat gaatgcaccc tgtcttcaca agaatatgtt catgaattac
601gatcgggtat atcagatgag aaacttctta attgcctaga atccctcagg gtttctttaa
661ccagcaatcc ggtcagctgg gttaacaact ttggccatga aggtcttgga ctcttattgg
721atgagctgga aaagcttctg gacaaaaaac agcaagaaaa tattgacaag aagaatcagt
781ataaacttat tcaatgcctc aaagcattta tgaataataa gtttggatta caaaggattc
841taggagatga aagaagtctt ttactattgg caagagcaat tgaccccaaa caacccaaca
901tgatgactga aatagtaaaa atactttctg ctatttgcat tgttggagaa gagaacattc
961tagataaact tttaggggct ataacaacag cagcagaaag aaataacagg gaacgatttt
1021caccaattgt ggaaggttta gaaaatcagg aagccttgca attacaggtg gcctgcatgc
1081agtttataaa tgcccttgtc acttctcctt atgagcttga ttttcgaata catttaagga
1141atgaattcct ccgttcagga ctaaaaacaa tgttaccaga tctaaaagaa aaagagaatg
1201atgagcttga tattcagttg aaagtatttg atgaaaacaa agaagatgac ctaactgaat
1261tatcacaccg tctcaatgac attcgagcag aaatggatga tatgaatgaa gtctaccatc
1321ttctatataa tatgctgaag gacactgctg ctgaaaatta cttcttatct attctacaac
1381attttttgct tatcagaaat gattattata tcaggccaca atattataaa ataattgagg
1441aatgtgtttc acagatagtg ctacactgca gtggtatgga tccagacttc aaatacaggc
1501aaagattaga catcgattta actcatctga tagattcttg tgtgaacaag gcgaaagttg
1561aagaaagtga acaaaaagct gcagagtttt caaagaagtt cgatgaagaa ttcacagctc
1621gacaggaagc tcaagcagag cttcaaaaaa gagatgagaa aatcaaagaa cttgaagcag
1681aaatccagca acttcgaacc caggcacaag tactctcaag ttcatcagga attccaggtc
1741ctcctgcagc acctccattg ccaggtgtag ggccgcctcc accaccaccc gcgccacctc
1801tacccggagg agctcctctt cctcctccac cacctccttt acctggaatg atggggatac
1861caccaccacc cccaccacca cttttatttg ggggacctcc tccaccacca ccccttggag
1921gagttcctcc tcccccagga atatcactta atctacctta tggaatgaag cagaaaaaaa
1981tgtataaacc tgaagtgtcc atgaagagaa tcaattggtc aaagattgaa cccacagaat
2041tatctgagaa ctgtttctgg ttaagagtca aagaagacaa gtttgagaat ccagatctct
2101ttgccaaatt ggcattgaat tttgctactc agataaaagt tcaaaagaac gcagaagcat
2161tagaagaaaa gaagactggg cctacaaaga agaaagtgaa agaactgaga attttggatc
2221ccaaaacagc tcagaatctg tccatctttc tgggatcata tcgcatgcca tatgaagaca
2281taagaaacgt tattctggag gttaatgaag acatgctgag tgaggcttta attcagaacc
2341ttgtgaaaca tcttcctgag cagaagatac tcaacgaatt agcagagctt aagaatgaat
2401atgatgacct ctgtgagcct gaacaatttg gagttgtgat gagctctgtg aaaatgttac
2461agcctcgtct cagtagtatc ctgttcaagc tcacatttga agaacacata aacaacatca
2521aaccaagcat catagcagta actcttgcct gtgaagaact gaagaaaagt gaaagcttta
2581acagactttt agagttagtt cttcttgttg gaaactacat gaactcaggc tcaagaaatg
2641cccagtcttt gggatttaag atcaacttcc tttgtaagat cagagatact aaatcagcgg
2701atcaaaaaac aacccttttg cattttattg ccgacatttg tgaggaaaaa tatcgagata
2761tcctaaaatt tcctgaagaa ctggaacacg tagaaagtgc aagcaaagtt tcagctcaaa
2821ttctcaagag caaccttgca tcaatggaac aacaaattgt tcatctggaa cgtgacatca
2881agaaattccc ccaagcagaa aatcaacacg ataagtttgt ggaaaagatg accagcttta
2941caaagactgc ccgagaacag tatgaaaaac tctccaccat gcacaacaac atgatgaagc
3001tctatgagaa tcttggagaa tacttcattt ttgactcaaa gacagtgagc atagaagagt
3061tctttggtga tctcaacaac ttccgaactt tgtttttgga agcagtgaga gaaaacaata
3121agagaagaga aatggaagag aagaccagga gggcaaaact tgcaaaagag aaagctgaac
3181aagaaaagtt agaacgccag aagaaaaaga aacaactcat tgatataaac aaagagggtg
3241atgagactgg tgtgatggat aatcttctag aagccctaca atcaggtgca gcattcagag
3301accgtcgaaa gcggattcca aggaatccag tggtaaatca tccctgtgca acaagggcta
3361atccaagatc agctacattg caaactttct tgtacaaagt ggttggtaag cctatcccta
3421accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
3481agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)